Clinical significance of lncRNA RP11-86H7.1 in the diagnosis of Kawasaki disease
PAN Lulu1, 2, LU Kongchang1, CHU Maoping1, ZENG Jingjing1, RONG Xing1
1.Children’s Heart Center, the Second Affiliated Hospital & Yuying Children’s Hospital, Institute of Cardiovascular Development & Translational Medicine, Wenzhou Medical University, Wenzhou 325027, China; 2.Department of Child Health Management, Wenzhou Women and Child Health Station,Wenzhou 325000, China
PAN Lulu,LU Kongchang,CHU Maoping, et al. Clinical significance of lncRNA RP11-86H7.1 in the diagnosis of Kawasaki disease[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2020, 50(8): 628-631，636.
Abstract：Objective: To investigate the expression of lncRNA RP11-86H7.1 in serum of Kawasaki disease (KD) patients and its correlation with clinicopathological characteristics and prognosis. Methods: The circulating
lncRNA specifically related to KD were screened and divided into four groups: children before KD treatment, children after KD treatment, children with common fever and healthy children group. The results of lncRNA
microarray showed that excluding the influence of fever, the expression of lncRNA in plasma of kawasaki patients was higher than that in normal children and during recovery period. The relative expression of serum lncRNA RP11-86H7.1 in the normal children group and the group before KD treatment was detected by real-time fluorescence quantitative PCR. The correlation between the expression of serum lncRNA RP11-86H7.1 and the clinicopathological characteristics of kawasaki disease was analyzed; ROC curve was drawn to analyze the diagnostic effect of serum lncRNA RP11-86H7.1 expression level on kawasaki disease. Results: The relative expression of serum lncRNA RP11-86 H7.1 in the children’s group before KD treatment was higher than that in the control group (P<0.05). There was no significant difference in age and sex ratio between the low-expression group and the low-expression group (P>0.05). Real-time PCR showed that the expression level of lncrnarp11-86H7.1 in serum of KD patients in acute stage was significantly higher than that in KD recovery stage, normal control group and fever control group. The differences were statistically significant (P<0.05). Conclusion: Serum lncRNA RP11-86H7.1 was up-regulated in patients with KD, and it can be used as a potential biomarker for early diagnosis and prognosis evaluation of KD.
