The study to the mouse models of hepatic non-alcoholic steatofibrosis
ZHOU Yuping1, 2, WEN Jinfeng1, 2, ZHOU Fei3, WANG Qingling3, YANG Ping4, LYU Xueyou1, 2
1.Department of Gastroenterology, the Affiliated Hospital of Medical School, Ningbo University, Ningbo 315020, China; 2.Institute of Digestive Disease, Ningbo University, Ningbo 315020, China; 3.Department of Biochemistry and Molecular Biology, Medical School of Ningbo University, Ningbo 315211, China; 4.School of Nursing, Ningbo College of Health Sciences, Ningbo 315100, China
ZHOU Yuping,WEN Jinfeng,ZHOU Fei, et al. The study to the mouse models of hepatic non-alcoholic steatofibrosis[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2020, 50(8): 603-608.
Abstract：Objective: To establish a hepatic non-alcoholic steatofibrosis (NASF) mouse model by the combination of methionine-choline-deficient diet (MCD) and repeated injection of carbon tetrachloride (CCl4). Methods: C57BL/6J mice were randomly divided into the control group and the model group. The model mice were fed with MCD, and intraperitoneally injected by 10% CCl4 once a week (2 mL/kg body weight), while the control group was fed with methionine-choline-supplemented diet (MCS) only. When all mice were killed at 4th and 6th weeksrespectively, the serum and the liver were collected. The histological analysis was conducted by HE staining, Oil Red O staining and Masson staining. The hepatic alpha smooth muscle ctin (α-SMA) protein expression was detected by immunohistochemistry. In addition, serum liver function and liver triglyceride levels were measured. Results: Compared with the control group, model group mice were significantly increased (P<0.01) in the activities of ALT, AST and TBIL in serum and the Triglyceride content in the liver. The liver tissue in model group mice showed obvious inflammation, steatosis and fibrosis. The α-SMA protein expression markedly increased, compared with the control group. Moreover, the pathological changes in the 6-week were more serious than those in the 4-week model mice. Conclusion: The NASF animal model can be established successfully by the combination of MCD and repeated injection of CCl4.
