DONG Jiaojiao,XIA Fangfang,JIN Zhousheng, et al. The effect of long chain fat emulsion on the myocardial ischemia reperfusion injury in rats[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2020, 50(7): 530-534.
Abstract：Objective: To investigate the protective effect of long chain fat emulsion on myocardial
ischemia/reperfusion (I/R) injury in rats. Methods: Twenty-one adult SD rats were randomly divided into three groups (n=7): sham operation group (Sham group), no drug treatment; model group (Model group), normal saline 2 mg·kg-1·d-1 injection for 5 days; long chain fat emulsion group (LE group), intralipid 2 mg·kg-1·d-1 injection for 5 days. Twenty-four hours after the last administration, the model of myocardial ischemia reperfusion (I/R) injury in rats was established by ligating the left anterior descending coronary. Hemodynamics was continuously monitored. The heart was taken for infarction area analysis, and the blood was collected for lactate dehydrogenase (LDH) detection. Results: Compared with the Model group, infarct size divided by area at risk (IR/AAR) and infarct size divided by left ventricle (IR/LV) were significantly reduced in LE group (all P<0.05), and LDH release was markedly lower than that in the Model group (P<0.05). The mean arterial pressure (MAP) and rate-pressure product (RPP) of rats in Model group were statistically lower than Sham group in ischemic period and reperfusion period (all P<0.05), while there was no significant difference between LE group and Sham group (P>0.05). Conclusion: Long chain fat emulsion pretreatment can reduce infarct area after myocardial I/R injury in rats, reduce LDH release and alleviate hemodynamic changes caused by I/R injury.
