1.Children’s Heart Center, the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou 325027, China; 2.Pediatric Research Institute, the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou 325027, China
CHEN Ruiyao1,RONG Xing,WU Tingting, et al. The effect of linc01635 on apoptosis of vascular endothelial cells in Kawasaki disease[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2020, 50(7): 525-529.
Abstract：Objective: To investigate the effect of linc01635 on apoptosis of vascular endothelial cells in Kawasaki disease (KD). Methods: Bioinformatics prediction and RNA FISH was employed to detect Subcellular localization of linc01635. Human umbilical vein endothelial cells (HUVECs) were cultured using KD plasma and healthy control plasma, qRT-PCR was used to detect the relative expression of linc01635 in HUVECs. Linc01635 expression in HUVECs was knocked-down by linc01635 siRNA and up-regulated by lentivirusor. Flow cytometry was used to analyze the apoptosis of HUVECs. Apoptosis was induced by KD plasma and the effect of linc01635 on KD-induced vascular endothelial cell injury was detected. Results: Linc01635 was distributed in both nuclei and cytoplasm of HUVECs. Compared with the healthy control group, KD plasma-treated HUVECs exhibited reduced expression of linc01635 (P<0.01). Overexpression of linc01635 inhibited HUVECs apoptosis (P<0.01), while knockdown of linc01635 increased HUVECs apoptosis (P<0.01). KD plasma could promote HUVECs apoptosis, and overexpression of linc01635 could inhibit KD plasma-induced apoptosis (P<0.01). Conclusion: KD plasma treatment can reduce the expression of linc01635 in vascular endothelial cells, which decreased linc01635 via KD plasma stimulation can induce vascular endothelial cells apoptosis.
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