结直肠癌组织浸润免疫细胞组成及特征

doi:10.3969/j.issn.2095-9400.2020.06.005

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摘要目的：研究结直肠癌（CRC）患者和健康人大肠组织中免疫细胞的浸润情况，为后续开展肿瘤微环境的研究提供前期的分析结果和数据。方法：通过GEO数据库对51例正常人结直肠组织和333例CRC患者肿瘤组织的基因表达谱数据进行计算分析，通过CIBERSORT方法将基因表达谱数据转化为组织中22种免疫细胞的浸润比例，对比这些免疫细胞在两者中的分布情况。结果：肠道浸润的免疫细胞组成在CRC患者与健康人之间有较大差异。T细胞和B细胞在肿瘤组织中的浸润数量少于健康人，而巨噬细胞、中性粒细胞、肥大细胞和树突状细胞在肿瘤组织中的浸润数量大于健康人。结论：CRC患者和健康人的结直肠组织中免疫细胞浸润存在较大的差异，这些具有差异的免疫细胞在癌症进展中可能发挥重要作用。

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	吴林峰
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关键词 ：结直肠癌, CIBERSORT, 肿瘤微环境, 免疫细胞

Abstract：Objective: To investigate the infiltrated immune cells in colorectal cancer patients and normal people, and to provide new ideas and directions for cancer related research. Methods: Gene expression profiles of 51 normal people and 333 colorectal cancer (CRC) patients from GEO database were analyzed, and CIBERSORT was used to transform the gene expression profile data into the infiltration ratio of 22 kinds of immune cells, and the distribution of these immune cells in both groupd of patients was compared. Results: The composition of intestinal infiltrated immune cells was significantly different between CRC patients and healthy people. The infiltration of T cells and B cells in tumor tissue was less than that in healthy people, while the infiltration of macrophages, neutrophils, mast cells and dendritic cells in tumor tissue was more than that in healthy people. Conclusion: There are great differences in the infiltration of immune cells in colorectal tissues between CRC patients and healthy people. These different immune cells may play an important role in the development of cancer.

Key words： colorectal cancer CIBERSORT tumor microenvironment immune cells

收稿日期: 2019-10-30

基金资助:国家自然科学基金资助项目（31670922，81672707，81602165）。

通讯作者: 沈淑蓉，副主任医师，Email：995477163@qq.com。

作者简介: 吴林峰（1985-），男，浙江温州人，主治医师，硕士。

链接本文:

https://xb.wmu.edu.cn/CN/10.3969/j.issn.2095-9400.2020.06.005 或 https://xb.wmu.edu.cn/CN/Y2020/V50/I6/454

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