YE Luxia,XU Lifei,XUE Yujie, et al. The protective effect of vitamin B12 on nerve cells damaged by oxidative stress[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2020, 50(6): 449-453.
Abstract：Objective: To explore the protective effect of Vitamin B12 pretreatment on PC12 cell injury induced by hydrogen peroxide and of its influence on autophagy and apoptotic protein expression. Methods: PC12 cells were divided as the control group (Control) with RPMI 1640 culturethe injury group (H2O2) with RPMI 1640 culture plus 200 μmol/L hydrogen peroxide stimulation and the Vitamin B12 protection group (H2O2+Vit B12) with RPMI 1640 culture plus 200 μmol/L vitamin B12 and 200 μmol/L hydrogen peroxide stimulation. Cell growth was examined 24 h later, and apoptosis and autophagy-related protein expression were detected by Western blot. Results: Compared with the Control group, the cell survival rate, the level of cell DNA replication, the expression level of Bcl-2 and P62 protein, the expression level of Bax, ATG-5 and LC3 protein, and the activation number of LC3 in cytoplasm increased significantly in the injury group (all P<0.05). Compared with the injury group, the cell survival rate and the level of cell DNA replication in the vitamin B12 protected group increased, the expression level of Bcl-2 and P62 protein increased, the expression level of Bax, ATG-5 and LC3 protein significantly decreased, and the activation number of LC3 in the cytoplasm decreased (all P<0.05). Conclusion: Vitamin B12 can inhibit oxidative stress-induced apoptosis by regulating autophagy.
