The effect of Hyp-PDT on K562 activity and autophagy pathway of leukemia cells cultured in vitro
WANG Dexuan1, WANG Wantie2, ZHENG Lyuzhen2, CHEN Weiwei1, YANG Qing1, ZHUANG Jieqiu1, XU Yixiao1, 2
1.Department of Pediatrics, the Second Affiliated & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou 325027, China; 2.Department of Pathophysiology, Wenzhou Medical University, Wenzhou 325035, China
WANG Dexuan,WANG Wantie,ZHENG Lyuzhen, et al. The effect of Hyp-PDT on K562 activity and autophagy pathway of leukemia cells cultured in vitro[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2020, 50(4): 285-289.
Abstract：Objective: To explore the effect of Hyp-PDT (Hypericin-mediated photodynamic therapy) on K562 activity and autophagy pathway of leukemia cells cultured in vitro. Methods: Human leukemia cell line K562 was cultured in vitro. Hyp-PDT was performed with 0.3 mW/cm2 light intensities and 4 min irradiation times. The CCK8 analysis was applied and the results were analyzed. Cell protein was collected instantly before irradiation, 4 h, 8 h and 16 h after irradiation respectively for Western blot determination of autophagy related protein. Morphological changes of K562 were observed under phase contrast microscope and electron microscopy. Results: Hyp-PDT with the light intensity of 0.3 mW/cm2 significantly decreased cell viabilities in K562 post irradiation at different time points (all P<0.01). After Hyp-PDT intervention, some cells in hypericin group showed swelling, degeneration and even dissolution after light irradiation. There were several cases of autophagy in K562 cells before irradiation, 8 h after irradiation, and autophagy increased in cells treated with DMSO but decreased obviously or even disappeared in Hyp group. The expression of LC3 protein decreased in Hyp group but increased slightly in DMSO group 8 h and 16 h after irradiation. The expression of Beclin-1 decreased 16 h after Hyp-PDT and a synchronous decrease of p-Akt protein was observed. Conclusion: Hyp-PDT of K562 in vitro can promote cell fatality by inhibiting autophagy in K562 cells. It may be related to the inhibition of Akt pathway.
