Ghrelin对大鼠胃排空及食管下括约肌压力的影响及其机制

doi:10.3969/j.issn.2095-9400.2020.02.015

摘要
图/表
参考文献
相关文章 (0)

全文: PDF (1341 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要目的：观察胃促生长素（Ghrelin）对大鼠胃排空及食管下括约肌（LES）压力的影响，同时测定血浆中胃动素和P物质水平的变化，探讨Ghrelin对胃排空及LES压力的影响及相关机制。方法：Wistar大鼠接受不同剂量Ghrelin（0.5、1.0、1.5、2.0、2.5 nmol/kg）腹腔注射后，测定胃排空率；Wistar大鼠接受1.5 nmol/kg Ghrelin腹腔注射后进行LES压力测定，放射免疫法测定血浆中胃动素及P物质浓度。结果：腹腔注射Ghrelin后大鼠胃排空率为38.24%±7.15%，与对照组（27.03%±2.91%）比明显加快（P＜0.01），同时该效应与腹腔注射Ghrelin的量呈量效关系，腹腔注射Ghrelin后大鼠LES压力[（19.57±3.26）mmHg]与对照组[（13.78±2.29）mmHg]比明显增加（P＜0.05），提示Ghrelin能增加大鼠LES压力；腹腔注射Ghrelin后大鼠血浆胃动素浓度[（217.28±25.38）ng/L]与对照组[（190.02±18.18）ng/L]比明显增加（P＜0.05），提示胃动素可能参与Ghrelin对大鼠食管下括约肌压力的调节。结论：中、高剂量Ghrelin腹腔注射，能明显加快健康大鼠的胃排空效应，Ghrelin能明显增加LES压力，胃动素可能参与Ghrelin对大鼠LES压力的调节。

	服务
	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	曹曙光
	吴昊
	林李淼

关键词 ：促生长素, 胃动素, 胃排空, 食管下括约肌压力

Abstract：Objective: To investigate the effects of Ghrelin on gastric emptying and lower esophageal sphincter pressure (LESP), expressions of Motilin and Substance P (SP) in plasma, and to explore the mechanism of Ghrelin on gastric emptying and LESP. Methods: After Wistar rats were intraperitioneally injected with different doses of Ghrelin (0.5, 1.0, 1.5, 2.0, 2.5 nmol/kg), gastric emptying rate was measured; after intraperitoneal injecion of 1.5 nmol/kg, LESP was measured, and plasma Motilin and SP concentrations were determined by radioimmunoassay. Results: After intraperitoneal injection of Ghrelin, gastric emptying rate was significantly faster than controls [(38.24±7.15)% vs. (27.03±2.91)%], and the accelerated gastric emptying had a significant dose-response relationship with Ghrelin. After intraperitoneal injection of Ghrelin, LESP [(19.57±3.26) mmHg] in rats was significantly increased compared with the control group [(13.78±2.29)mmHg] (P<0.05), suggested that Ghrelin could increase the LESP in rats. After intraperitoneal injection of Ghrelin, plasma Motilin concentration [(217.28±25.38)ng/L] in rats was significantly increased compared with the control group [(190.02±18.18)ng/L] (P<0.05), suggesting that Motilin may be involved in the LESP regulation in rats. Conclusion: Intraperitoneal injection of Ghrelin can accelerate gastric emptying in rats, Ghrelin may increase the pressure of the lower esophageal sphincter in rats, and Motilin may be involved in the LESP regulation.

Key words： Ghrelin Motilin gastric emptying lower esophageal sphincter pressure

基金资助:温州市科技计划项目（Y20150166）。

作者简介: 曹曙光（1975-），男，浙江瑞安人，副主任医师，博士。

链接本文:

https://xb.wmu.edu.cn/CN/10.3969/j.issn.2095-9400.2020.02.015 或 https://xb.wmu.edu.cn/CN/Y2020/V50/I2/158

[1] KAMEGAI J, TAMURA H, SHIMIZU T, et al. Chronic central infusion of ghrelin increases hypothalamic neuropeptide Y and Agouti-related protein mRNA levels and body weight in rats[J]. Diabetes, 2001, 50(11): 2438-2443.
[2] LV Y, LIANG T, WANG G, et al. Ghrelin, a gastrointestinal hormone, regulates energy balance and lipid metabolism[J].Biosci Rep, 2018, 38(5). pii: BSR20181061.
[3] ASAKAWA A, INUI A, KAGA T, et al. Ghrelin is an appetite-stimulatory signal from stomach with structural resemblance to motilin[J]. Gastroenterology, 2001, 120(2): 337-345.
[4] 孙玉舫, 王松坡, 蒋振明, 等. 调气活血中药对大鼠慢性萎缩性胃炎病理改变及胃排空的影响[J]. 上海中医药杂志, 2009, 43(7): 64-66.
[5] KOUTOURATSAS T, KALLI T, KARAMANOLIS G, et al.
Contribution of ghrelin to functional gastrointestinal disorders’ pathogenesis[J]. World J Gastroenterol, 2019, 25(5): 539-551.
[6] KITAZAWA T, DE SMET B, VERBEKE K, et al. Gastric motor effects of peptide and non-peptide ghrelin agonists in mice in vivo and in vitro[J]. Gut, 2005, 54(8): 1078-1084.
[7] POITRAS P, POLVINO W J, ROCHELEAU B, et al. Gastrokinetic effect of ghrelin analog RC-1139 in the rat. Effect on post-operative and on morphine induced ileus[J]. Peptides, 2005, 26(9): 1598-1601.
[8] DORNONVILLE DE LA COUR C, LINDSTRÖM E, NORLÉN P, et al. Ghrelin stimulates gastric emptying but is without effect on acid secretion and gastric endocrine cells[J].Regul Pept, 2004, 120(1-3): 23-32
[9] NELSON A D, CAMILLERI M, ACOSTA A, et al. Effects of ghrelin receptor agonist, relamorelin, on gastric motor functions and satiation in healthy volunteers[J]. Neurogastroenterol Motil, 2016, 28(11): 1705-1713.
[10] TACK J, DEPOORTERE I, BISSCHOPS R, et al. Influence of ghrelin on gastric emptying and meal-related symptoms in idiopathic gastroparesis[J]. Aliment Pharmacol Ther, 2005, 22(9): 847-853.
[11] TACK J, DEPOORTERE I, BISSCHOPS R, et al. Influence of ghrelin on interdigestive gastrointestinal motility in hu-mans[J]. Gut, 2006, 55(3): 327-333
[12] HUANG H H, CHEN L Y, DOONG M L, et al. α-melanocyte
stimulating hormone modulates the central acyl ghrelin-induced stimulation of feeding, gastrointestinal motility, and colonic secretion[J]. Drug Des Devel Ther, 2017, 11: 2377-2386.
[13] GALLAS S, SINNO M H, BOUKHETTALA N, et al. Gastric electrical stimulation increases ghrelin production and inhibits catecholaminergic brainstem neurons in rats[J]. Eur J Neurosci, 2011, 33(2): 276-284.
[14] WO J M, EJSKJAER N, HELLSTRÖM P M, et al. Randomised clinical trial: ghrelin agonist TZP-101 relieves gastroparesis associated with severe nausea and vomiting--randomised clinical study subset data[J]. Aliment Pharmacol Ther, 2011, 33(6): 679-688.
[15] WANG L, MURPHY N P, STENGEL A, et al. Ghrelin prevents levodopa-induced inhibition of gastric emptying and increases circulating levodopa in fasted rats[J]. Neurogastroenterol Motil, 2012, 24(5): 235-245.
[16] KARASAWA H, PIETRA C, GIULIANO C, et al. New ghrelin agonist, HM01 alleviates constipation and L-dopa-delayed gastric emptying in 6-hydroxydopamine rat model of Parkinson’s disease[J]. Neurogastroenterol Motil, 2014, 26(12): 1771-1782.
[17] HERCULANO J R Jr, TRONCON L E, APRILE L R, et al. Diminished retention of food in the proximal stomach correlates with increased acidic reflux in patients with gastroesophageal reflux disease and dyspeptic symptoms[J]. Dig Dis Sci, 2004, 49(5): 750-756.
[18] MIWA H, KOSEKI J, OSHIMA T, et al. Rikkunshito, a traditional Japanese medicine, may relieve abdominal symptoms in rats with experimental esophagitis by improving the barrier function of epithelial cells in esophagealmucosa[J]. J Gastroenterol, 2010, 45(5): 478-487.
[19] LEE H L, EUN C S, LEE O Y, et al. Association between GERD-related erosive esophagitis and obesity[J]. J Clin Gastroenterol, 2008, 42(6): 672-675.
[20] EL-SERAG H B, ERGUN G A, PANDOLFINO J, et al. Obesity increases oesophageal acid exposure[J]. Gut, 2007, 56(6): 749-755.
[21] THOMAS S J, ALMERS L, SCHNEIDER J, et al. Ghrelin and leptin have a complex relationship with risk of barrett's esophagus[J]. Dig Dis Sci, 2016, 61(1): 70-79.