A型肉毒素对带状疱疹后神经痛小鼠炎性神经递质表达的影响

doi:10.3969/j.issn.2095-9400.2020.01.010

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摘要目的：观察A型肉毒素（BTX-A）对带状疱疹后神经痛（PHN）小鼠炎性神经递质表达的影响，探讨其可能的作用机制。方法：将36只PHN雄性C57小鼠随机分成A、B、C 3组，每组12只。A组在疱疹部位注射0.9%氯化钠溶液；B组在疱疹部位注射BTX-A（2 U/kg）；C组在L5背根神经节（DRG）处注射BTX-A（2 U/kg）。连续干预7 d后处死小鼠，取小鼠DRG和相应的脊髓节段，采用RT-qPCR和ELISA法检测各组小鼠DRG和脊髓中炎性神经递质TNF-α、IL-1β、神经激肽A的mRNA表达，采用Western blot检测各组小鼠DRG和脊髓中Na1.3、Na1.8通道蛋白表达。结果：RT-qPCR检测结果显示，与A组比，B组和C组炎性神经递质TNF-α、IL-1β、神经激肽A的mRNA表达水平显著降低（P＜0.05），但B和C 2组间比较差异无统计学意义。ELISA法检测结果显示，与A组比，B组和C组TNF-α、IL-1β、神经激肽A的蛋白含量显著降低（P＜0.05），但B和C 2组间比较差异无统计学意义。Western blot结果显示，与A组比，B组和C组Na1.3、Na1.8通道蛋白表达显著降低（P＜0.05），但B和C 2组间比较差异无统计学意义。结论：BTX-A能有效抑制PHN，其作用机制可能是通过降低通道蛋白Na1.3、Na1.8的表达，抑制炎性神经递质TNF-α、IL-1β、神经激肽A的mRNA表达水平和蛋白含量。

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	李国松
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关键词 ： A型肉毒素, 带状疱疹后神经痛, 炎性神经递质, 小鼠

Abstract：Objective: To explore the mechanism of botulinum toxin A (BTX-A) on postherpetic neuralgia (PHN) by observing its effect on the expression of inflammatory neurotransmitter in mice with PNH. Methods: Thirty-six male C57 mice with PHN were randomly divided as group A, B and C, with 12 mice in each group. Herpes site in group A was injected with 0.9% saline, with BTX-A (2 U/kg) in group B, while BTX-A (2 U/kg) was injected into dorsal root ganglion (DRG) of the fifth lumber spine in group C. All mice were sacrificed after 7 d of continuous intervention, and DRG and corresponding segments of spinal cord were isolated from mice. The mRNA expression levels of TNF-α, IL-1β and neurokinin A in DRG and spinal cord was detected by RT-qPCR and ELISA. The expression of Na1.3 and Na1.8 channels in DRG and spinal cord was detected by Western blot. Results: RT-qPCR results showed that the mRNA expression levels of inflammatory neurotransmitters on TNF-α, IL-1β and neurokinin A in group B and group C were significantly lower than those in group A (P<0.05), but there was no significant difference between group B and group C. The results of ELISA showed that the protein contents of TNF-α, IL-1β and neurokinin A in group B and group C were significantly lower than those in group A (P<0.05), but there was no significant difference between group B and group C. Western blot results showed that the expression of Na1.3 and Na1.8 channel proteins in group B and group C decreased significantly compared with group A (P<0.05), but there was no statistical difference between the two groups. Conclusion: BTX-A can effectively inhibit PHN, and its mechanism may be by reducing the expression of Na1.3 and Na1.8 channel proteins and thus inhibiting the expression of inflammatory neurotransmitters TNF-α, IL-1β and neurokinin A.

Key words： botulinum toxin A postherpetic neuralgia inflammatory neurotransmitter mice

收稿日期: 2019-06-15

基金资助:杭州市科技计划项目（20171226Y95）。

通讯作者: 汤样华，副主任医师，Email：tangyanghua168@163.com。

作者简介: 李国松（1976-），男，浙江杭州人，副主任医师。

链接本文:

https://xb.wmu.edu.cn/CN/ 10.3969/j.issn.2095-9400.2020.01.010 或 https://xb.wmu.edu.cn/CN/Y2020/V50/I1/51

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