FU Chao,MEI Tingting,WANG Hongtao, et al. The association of CD4+, CD8+ T lymphocytes in colorectal cancer with postoperative prognosis[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2020, 50(1): 30-35.
Abstract：Objective: To investigate the association of CD4+, CD8+ T lymphocytes in colorectal cancer with postoperative prognosis. Methods: Clinical data and resection samples of 109 colorectal cancer patients identified by pathology undergoing resection in our department from January 2012 to January 2014 were collected. The CD4+, CD8+ T lymphocytes in colorectal cancer were detected by immunohistochemical staining and grouped according to the high and low levels. Kaplan-Meier survival curve analysis was used to compare clinical features and prognosis. The Cox proportional hazard regression model was used to analyze the clinical factors that may affect the prognosis of patients with univariate and multivariate analysis. Results: Immunohistochemical staining showed that CD8+ T cell infiltration was high in 78 cases, low in 31 cases; CD4+ T cell infiltration was high in 56 cases, and low level in 53 cases. The levels of CD4+ and CD8+ T lymphocytes in colorectal cancer tissues were closely related to tumor stage and depth of invasion (all P<0.05). Follow-up analysis showed that colorectal cancer patients with high levels of CD8+ and their CD8+/CD4+ had a better prognosis for DFS and OS (P<0.05). And there was no significant difference between DFS, OS and low-level group in patients with high-level CD4+ colorectal cancer. Cox regression model analysis showed that tumor stage, lymph node metastasis, CD8+ and CD8+/CD4+ were independent risk factors for DFS and OS prognosis in patients with colorectal cancer (P<0.05). Conclusion: The level of CD8+ and CD8+/CD4+ could be a good reference value for the prognosis of colorectal cancer patients.
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