miR-144-5p、miR-668-3p、miR-134-5p在儿童原发性免疫性血小板减少症中的表达

doi:10.3969/j.issn.2095-9400.2020.01.004

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摘要目的：探讨外周血单个核细胞（PBMCs）中miR-144-5p、miR-668-3p、miR-134-5p在儿童原发性免疫性血小板减少症（ITP）中的表达变化。方法：采用RT-qPCR检测25例ITP患儿和15例正常对照PBMCs中3种miRNAs相对表达量，Sysmex XE-5000全自动血细胞分析仪检测血小板计数及未成熟血小板分数（IPF%）；分析miRNAs与血小板计数的相关性，ROC曲线分析检验效能。结果：ITP患儿组PBMCs中的miR-144-5p和miR-668-3p较对照组表达均上调，miR-134-5p表达下调（均P＜0.05）。miR-144-5p和miR-668-3p在急性ITP患儿组中表达量要高于慢性ITP患儿组（均P＜0.05），且miR-144-5P（r=-0.802，P＜0.001）和miR-668-3p（r=-0.753，P＜0.001）的相对表达量与外周血小板计数呈负相关。相较于单个指标，miR-144-5p联合IPF%诊断急性ITP的AUC为0.960，灵敏度和特异度分别为93.3%和100%；而miR-134-5p联合IPF%诊断慢性ITP的AUC为0.967，灵敏度和特异度分别为90.0%和93.3%。结论：miR-144-5p和miR-668-3p在ITP患儿PBMCs中表达显著上调，miR-134-5p表达下调，这些改变可能在疾病的发生与发展中发挥了重要作用。

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	金速速
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	舒旷怡
	李姗姗
	柳洁
	江明华

关键词 ：免疫性血小板减少症, 微小RNA, 未成熟血小板, 实时荧光定量聚合酶链反应

Abstract：Objective: To investigate the changes of microRNAs (miR-144-5p, miR-668-3p and miR-134-5p) in peripheral blood mononuclear cells (PBMCs) in children with primary immune thrombocytopenia (ITP). Methods: Real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) was used to detect the relative expression of the three miRNAs from 25 ITP patients and healthy control in PBMCs. Platelet count and immature platelet fraction (IPF%) were detected by Sysmex XE-5000 automatic blood analyzer. The correlations of the miRNAs expression with peripheral platelet count were analyzed and diagnostic value was measured by receiver operating characteristic curve. Results: Compared with the normal control group, the expressions of miR-144-5p and miR-668-3p of the ITP group in PBMCs were significantly up-regulated (P<0.05), and were negatively correlated with platelet count (r=-0.802, P<0.001; r=-0.753, P<0.001), while the expressions of miR-134-5p was down-regulated significantly (P<0.05). The expressions of miR-144-5p and miR-668-3p in the acute ITP group were higher than those in the chronic ITP group (P<0.05). In terms of the single indicator, the AUC of miR-144-5p combined with IPF% in the diagnosis of acute ITP was 0.960. The sensitivity and specificity were 93.3% and 100% respectively. The AUC of miR-134-5p combined with IPF% in the diagnosis of chronic ITP was 0.967. The sensitivity and specificity were 90.0% and 93.3% respectively. Conclusion: miR-144-5p and miR-668-3p in PBMCs were significantly up-regulated and miR-134-5p was down-regulated in children with ITP, which suggests they may play an important role in the incidence and development of ITP.

Key words： immune thrombocytopenia microRNA immature platelet fraction quantitative real-time polymerase chain reaction

收稿日期: 2019-05-15

基金资助:浙江省医药卫生科技计划项目（2016RCA023）；温州市公益性科技计划项目（Y20160161，Y20190227）。

通讯作者: 江明华，主任技师，Email：minghua93@126.com。

作者简介: 金速速（1989-），女，浙江温州人，主管技师，在职硕士生。

链接本文:

https://xb.wmu.edu.cn/CN/10.3969/j.issn.2095-9400.2020.01.004 或 https://xb.wmu.edu.cn/CN/Y2020/V50/I1/19

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