扶正中药逆转TLR4过表达乳腺癌紫杉醇耐药的机制

doi:10.3969/j.issn.2095-9400.2019.11.008

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摘要目的：探讨扶正中药联合紫杉醇（PTX）逆转TLR4过表达乳腺癌细胞株MDA-MB-231对PTX的耐药及对TLR4/NF-κB信号通路的影响。方法：MTT实验检测扶正中药、PTX及两药联用对乳腺癌细胞株T47D及MDA-MB-231的增殖抑制作用；流式细胞技术检测细胞凋亡率，Western blot检测细胞凋亡相关蛋白及TLR4/NF-κB信号通路相关蛋白的表达。结果：扶正中药与PTX在处理MDA-MB-231细胞株时有协同作用，可诱导MDA-MB-231细胞株细胞凋亡增加（P＜0.05），使TLR4/NF-κB信号通路相关蛋白TLR4、p-lκBα、NF-κB表达减少（P＜0.05）。结论：MDA-MB-231细胞株中，扶正中药可以通过抑制TLR4/NF-κB信号通路增加细胞凋亡，从而增加PTX对MDA-MB-231乳腺癌细胞株的增殖抑制作用，逆转乳腺癌对PTX的耐药。

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	张祥建
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关键词 ：扶正中药；紫杉醇；耐药；乳腺癌细胞株；TLR4/NF-&kappa, B信号通路

Abstract：Objective: To investigate the mechanism of Fuzheng Chinese medicine combined with paclitaxel against TLR4 over-expressing breast cancer, and to confirm the sensitization effect of traditional Chinese medicine combined with chemotherapy for TLR4 over-expressed breast cancer MDA-MB-231 so as to select specific tumor phenotype for combination of traditional Chinese and western medicine. The target provides drug intervention to provide basic data. Methods: MTT assay was used to detect the proliferation inhibition effect of Fuzheng Chinese medicine, paclitaxel and two drugs on breast cancer cell lines T47D and MDA-MB-231. The apoptosis rate was detected by flow cytometry. Expression of the apoptosis related protein and TLR4/NF-kB signaling pathway-associated proteins was detected by Western blotting technology. Results: Fuzheng Chinese medicine and paclitaxel had synergistic effect in the treatment of MDA-MB-231 cell line; Fuzheng Chinese medicine assisted paclitaxel to induce apoptosis of MDA-MB-231 cell line (P<0.05); combined with Chinese medicine and paclitaxel, MDA-MB-231 Cell line TLR4/NF-kB signaling pathway was inhibited (P<0.05). Conclusion: In MDA-MB-231 cell line, Fuzheng Chinese medicine can increase cell apoptosis by inhibiting TLR4/NF-kB signaling pathway, thereby increasing the cell proliferation inhibition effect of paclitaxel on MDA-MB-231 breast cancer cell line and reversing paclitaxel-resistance in breast cancer.

Key words： Fuzheng Chinese medicine paclitaxel drug resistance breast cancer cell line TLR4/NF-kB signaling pathway

收稿日期: 2019-05-24

基金资助:温州市科技计划项目（Y20170182）。

作者简介: 张祥建（1990-），男，浙江温州人，住院医师，硕士。

链接本文:

https://xb.wmu.edu.cn/CN/10.3969/j.issn.2095-9400.2019.11.008 或 https://xb.wmu.edu.cn/CN/Y2019/V49/I11/826

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