Expression and localization of estrogen receptor-β in nucleus pulposus cells of the human intervertebral disc
1.Department of Orthopaedics, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou ,
325027; 2.The Second School of Clinical Medicine, Wenzhou Medical University, Wenzhou, 325035
CHEN Hongliang1,KE Xiurong2,LOU Chao1, et al. Expression and localization of estrogen receptor-β in nucleus pulposus cells of the human intervertebral disc[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2014, 44(9): 646-.
Abstract：Objective: To investigate the expression of estrogen receptor-β in nucleus pulposus cells of the human intervertebral disc. Methods: Seven nucleus pulposus specimens were obtained from 7 patients during surgery. Cultured disc cells were used for immunocytochemical localization of ERβ. mRNA was isolated from cultured human disc cells, and RT-PCR amplification of ERβ was employed to document molecular expression of this receptor. Results: Expression and localization of estrogen receptor-β were detected in all 7 specimens. A goldview-stained agarose gel electrophoresis showing gene expression of ERβ as a single band at the predicted 323 bp size. Localization was seen in the cytoplasm in all cultured disc cells with the immunocytochemistry method. Conclusion: This study demonstrates the expression of estrogen receptor-β in nucleus pulposus cells of the human intervertebral disc
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