Preparation and biocompatibility of kidney acellular matrix in rats as tissue engineering scaffold
1.Department of Anatomy, Wenzhou Medical University, Wenzhou, 325035; 2.Forensic Department of Public Security Burreau, Qichun, 435300; 3.Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou, 325035
DAI Kaiyu1,YI Xudong2,ZHAO Lina1, et al. Preparation and biocompatibility of kidney acellular matrix in rats as tissue engineering scaffold[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2014, 44(9): 641-.
Abstract：Objective: To prepare the kidney decellularized matrix bio-derived scaffold with lower antigenicity, complete protein components and good biological properties in rats and evaluate its biocompatibility in vivo. Methods: By the perfusion with chemical detergents through the abdominal aorta, the acellular scaffolds of kidney were prepared in SD rats. Then the decellularized scaffolds and native kidneys were analyzed through genomic DNA content analysis, transmission electron microscopy (TEM), HE, PAS, Massons and immunofluorescence. Acellular scaffolds were transplanted in situ to evaluate the biocompatibility. Results: After being treated by a variety of reagents, cells within kidney tissue had been largely removed. The acellular scaffold had forms of three-dimensional network structure with integrated vascular network. DNA contents of the acellular renal ECM in the experimental group were 3%, less than that in the normal group. Immunohistochemistry showed a lot of collagen fibers and no significant residual cell debris within scaffold was found. The gross and histological observation of subdermal implantation showed the inflammatory reactions were severe onset. There was an increased collagen fiber density and angiogenesis, and the transplanted acellular matrix was gradually degraded and absorbed. Conclusion: The renal scaffolds prepared in rats are acellular but have the structure of three-dimensional network. The reticular structure of the extracellular matrix is well preserved, which has good biocompatibility in vivo as tissue engineering scaffold for renal reconstruction and regeneration
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