亮氨酸对大鼠肝部分切除术后肝再生的影响

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摘要目的：观察不同亮氨酸浓度的氨基酸配方对大鼠肝部分切除术后残肝再生能力的影响。方法：18只SD大鼠行70%肝切除术后，经胃造瘘管给予不同亮氨酸浓度的氨基酸配方（1 g/kg），分别为平衡氨基酸、支链氨基酸、亮氨酸，7 d后检测残肝/体质量比、肝细胞Ki-67和PCNA阳性计数。结果：以平衡氨基酸组为对照，亮氨酸组的残肝/体质量比、Ki-67和PCNA阳性计数均有显著增加，且要高于支链氨基酸组（P＜0.05）；支链氨基酸组的Ki-67和PCNA阳性计数较平衡氨基酸组有显著增加（P＜0.05），但残肝/体质量比差异无统计学意义。结论：亮氨酸有利于肝部分切除大鼠残肝的再生，其作用优于支链氨基酸。

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	林建华
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关键词 ：亮氨酸, 支链氨基酸, 肝切除术, 肝再生, 大鼠

Abstract：Objective: To investigate the effect of amino acid solutions with different leucine concentrations on liver regeneration after partial hepatectomy in rats. Methods: After 70% hepatectomy, eighteen SD rats were fed via gastric tube with one of the following amino acid solutions (1 g/kg): balanced amino acid (BAA group), branched chain amino acid (BCAA group) and leucine (Leu group). They were different in leucine concentrations. Liver regeneration was determined by residual liver/body weight ratio, ki-67 and PCNA positive counts 7 days later. Results: Compared with BAA group, the residual liver/body weight ratio, ki-67 and PCNA positive counts in Leu group were significantly increased (P<0.05), and were higher than those in BCAA group. Ki-67 and PCNA positive counts in BCAA group were significantly increased too when compared to BAA group, but residual liver/body weight ratio showed no significant difference between the two groups. Conclusion: Leucine can promote liver regeneration after partial hepatectomy in rats, and is more effective than branched chain amino acid.

Key words： leucine branched chain amino acid hepatectomy liver regeneration rats

收稿日期: 2019-02-25

基金资助:温州市科技计划项目（Y20140398）。

通讯作者: 周斌，副主任医师，Email：pzhoubin@sina.com。

作者简介: 林建华（1982-），男，浙江瑞安人，主治医师，硕士。

链接本文:

https://xb.wmu.edu.cn/CN/ 或 https://xb.wmu.edu.cn/CN/Y2019/V49/I10/753

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