长链非编码RNA MIAT通过miR-124促进鼻咽癌细胞增殖、迁移和侵袭

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摘要目的：探究长链非编码RNA MIAT是否通过miR-124调节鼻咽癌细胞增殖、迁移与侵袭。方法：采用荧光定量PCR检测鼻咽癌细胞中MIAT水平，通过检测荧光素酶活性评估MIAT和miR-124之间的相互作用。随后将si-MIAT与miR-124 inhibitor分别或同时转染鼻咽癌细胞，检测其对鼻咽癌细胞生长、迁移与侵袭的影响。采用Western blot检测Wnt/β-catenin信号通路蛋白的表达情况。结果：与正常鼻咽上皮细胞NP-69相比，鼻咽癌细胞HONE-1中MIAT的表达水平显著提高。在线软件与双荧光素酶活性实验分析证实MIAT与miR-124之间存在相互作用。进一步实验表明，si-MIAT可上调miR-124水平，从而抑制鼻咽癌细胞增殖、迁移与侵袭。Western blot分析显示si-MIAT通过miR-124抑制Wnt/β-catenin信号通路在鼻咽癌细胞中发挥促癌作用。结论：MIAT通过miR-124调节Wnt/β-catenin信号通路进而影响鼻咽癌细胞增殖、迁移与侵袭。MIAT靶向调控miR-124，可作为鼻咽癌诊断与治疗的一个潜在靶点。

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	郝亚琳
	金晓杰
	赵辉
	何平
	张佳凤
	董琼娜
	施薇薇
	赵苗苗

关键词 ： RNA, 长链非编码, MIAT, miR-124, 鼻咽癌

Abstract：Objective: To investigate whether long non-coding RNA MIAT regulates cell proliferation, migration and invasion in nasopharyngeal carcinoma cells through miR-124. Methods: qRT-qPCR was performed to detect MIAT expression in nasopharyngeal carcinoma cells. The interaction between MIAT and miR-124 was evaluated by dual luciferase reporter assay. si-MIAT and miR-124 inhibitor were transfected into nasopharyngeal carcinoma cells to detect their effects on proliferation, migration and invasion of nasopharyngeal carcinoma cells. The expression of Wnt/β-catenin signaling pathway was determined by Western blot. Results: Compared with normal nasopharyngeal epithelial cells NP-69, MIAT was significantly upregulated in nasopharyngeal carcinoma cell HONE-1. The online software and luciferase activity assay confirmed the interaction between MIAT and miR-124. Further experiments indicated that si-MIAT could increase the expression of miR-124, thus inhibiting cell proliferation, migration and invasion of nasopharyngeal carcinoma. The results of Western blot showed that MIAT functioned as an oncogene in nasopharyngeal carcinoma cell by up-regulating Wnt/β-catenin signaling pathway through miR-124. Conclusion: Our results demonstrated that MIAT affected proliferation, migration and invasion of nasopharyngeal carcinoma cells by regulating the expression of Wnt/β-catenin signaling pathway via miR-124. MIAT was a potential target for the diagnosis and treatment of nasopharyngeal carcinoma.

Key words： RNA, long non-coding MIAT miR-124 nasopharyngeal carcinoma

收稿日期: 2019-02-05

通讯作者: 金晓杰，主任医师，Email：jinxiaojie2@126.com。

作者简介: 郝亚琳（1986-），女，河南周口人，住院医师，硕士。

链接本文:

https://xb.wmu.edu.cn/CN/ 或 https://xb.wmu.edu.cn/CN/Y2019/V49/I10/718

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