电针对慢性应激抑郁模型大鼠海马突触可塑性蛋白的影响

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摘要目的：探讨电针对慢性应激抑郁模型大鼠海马突触性蛋白表达的影响，明确其治疗机制。方法：48只SD大鼠随机分为4组：对照组、模型组、电针组、氟西汀组，除对照组外，各组均采用慢性不可预见性温和应激（CUMS）建立抑郁动物模型。电针组于每日应激前选取百会、印堂两穴进行电针治疗，每天1次；氟西汀组于造模前通过灌胃给予阳性药氟西汀水溶液，剂量为10 mg/kg，持续21 d。于应激造模后7、14、21 d分别采用旷场测试和强迫游泳实验评价大鼠的抑郁样行为，Golgi染色观察海马突触结构的病理特点，Western blot检测海马突触可塑性相关蛋白SYN、PSD-95、CREB、BDNF的表达。结果：与对照组比，模型组大鼠7 d时强迫游泳不动时间显著增加（P＜0.05），14 d时旷场自主活动评分显著下降（P＜0.05），抑郁样行为明显，海马突触结构损伤，突触可塑性蛋白SYN、PSD-95、CREB、BDNF表达均显著下调（P＜0.01）；经电针治疗后，大鼠抑郁样行为得以明显改善（P＜0.01），树突棘形态及数量趋于正常，SYN、PSD-95、CREB、BDNF表达均明显回升（P＜0.01）。结论：电针能显著改善慢性应激抑郁模型大鼠的抑郁样行为，其机制与改善突触结构，上调突触可塑性蛋白表达有关。

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	叶必宏
	刘海飞
	李灵浙
	潘胜莲
	宋丰军
	王庆来
	朱文宗

关键词 ：电针, 抑郁, 慢性应激, 海马, 突触可塑性, 大鼠

Abstract：Objective: To investigate the effects of electroacupuncture on the expression of synaptic protein in hippocampus of chronic stress depression rats, and to clarify its therapeutic mechanism. Methods: Forty-eight SD rats were randomly divided into 4 groups: blank control group, model group, electroacupuncture group and fluoxetine group. In addition to the control group, chronic unpredictable mild stress (CUMS) was used to establish a model of depression. The electroacupuncture group was treated with electroacupuncture once a day at the two points of “Baihui” and “Yintang” before daily stress; the fluoxetine group was given a positive drug fluoxetine solution before the modeling, with the dose of 10 mg/kg for 21 d. On the 7th, 14th and 21st day of stress modeling, the depression-like behaviors of rats were evaluated by open field test and forced swimming test. The pathological features of hippocampal synaptic structure were observed by Golgi staining. The hippocampal synaptic plasticity protein SYN, PSD-95, CREB and BDNF was detected by Western-blotting test. Results: Compared with the control group, the forced swimming time of the model group was significantly increased at 7 d (P<0.05), and the spontaneous activity of the open field was significantly decreased at 14 d (P<0.05), and the depression-like behavior was obvious. The structural damage, synaptic plasticity protein SYN, PSD-95, CREB, BDNF expression were significantly down-regulated (P<0.01); after electroacupuncture treatment, the depression-like behavior of rats was significantly improved (P<0.01), dendritic spine morphology The number of SYN, PSD-95, CREB and BDNF increased significantly (P<0.01). Conclusion: Electroacupuncture can significantly improve the depression-like behavior of chronic stress-depressive rats, which is related to the improvement of synaptic structure and up-regulation of synaptic plasticity protein expression.

Key words： electroacupuncture depression chronic stress hippocampus synaptic plasticity rats

收稿日期: 2019-03-22

基金资助:“十三五”浙江省中医药重点学科（2017-xk-A49）。

作者简介: 叶必宏（1985-），男，浙江温州人，主治医师，硕士。

链接本文:

https://xb.wmu.edu.cn/CN/ 或 https://xb.wmu.edu.cn/CN/Y2019/V49/I9/673

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