锌对邻苯二甲酸酯类暴露致雄性生殖毒性的干预作用

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摘要目的：研究锌对邻苯二甲酸酯类（PEs）暴露致雄性小鼠生殖毒性的干预作用。方法：36只雄性ICR小鼠随机分对照组，邻苯二甲酸丁苄酯（BBP）、邻苯二甲酸正二丁酯（DBP）、邻苯二甲酸二（2-乙基己基）酯（DEHP）3种邻苯二甲酸酯类（PEs）等毒性混合物（MIXPs）暴露组（MIXPs组）以及MIXPs+ZnSO4组。MIXPs组小鼠经口灌胃MIXPs 160 mg·kg-1·d-1，MIXPs+ZnSO4组小鼠同时经口灌胃MIXPs 160 mg·kg-1·d-1+ZnSO4 50 mg·kg-1·d-1，对照组小鼠灌胃等容量的赋形剂（0.5% CMC-Na）。连续干预90 d，眶静脉取血后、取睾丸和附睾等雄性生殖器官，观察精子形态，试剂盒测定睾酮、SOD、MDA、T-AOC等水平。结果：与对照组相比，MIXPs组小鼠体质量显著降低，肛殖距缩短（均P＜0.05）；血清睾酮水平显著降低，精子畸形率显著升高，睾丸组织中SOD、MDA显著改变（均P＜0.01）；而MIXPs+ZnSO4组与对照组差异无统计学意义（P＞0.05）。结论：锌可抑制MIXPs暴露诱导小鼠雄性生殖毒性，可能与其改善睾丸组织的抗氧化水平，提高血清睾酮水平有关。

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	孙悦
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	高海涛

关键词 ：锌, 邻苯二甲酸酯类, 雄性生殖毒性, 抗氧化, 小鼠

Abstract：Objective: To study the protective effect of zinc on reproductive toxicity caused by phthalate exposure in mice. Methods: Thirty-six male ICR mice were randomly divided into the control group, MIXPs (the equipotent toxic mixture of butyl benzyl phthalate (BBP), di (n-butyl) phthalate (DBP) and di (2-ethyhexyl) phthalate (DEHP) group, and MIXPs+ZnSO4 group, with 12 mice in each group. Mice in MIXPs group and MIXPs+ZnSO4 group were treated with 160 mg·kg-1·d-1 MIXPs by gavage with or without 50 mg·kg-1·d-1 ZnSO4. Mice in control group were treated with isodose 0.5% CMC-Na. After 90 days’ intervention, orbital vein blood was collected from each anesthetic mouse. Then dissect the mouse and take male reproductive organs such as the testicles and epididymis. Sperm morphology was observed with electron microscope. The levels of testosterone, SOD, MDA and T-AOC were measured with the commercial kits. Results: Compared with the control, the bodyweight, anogenital distance, serum testosterone, SOD and MDA in testicular tissue in MIXPs group were significantly reduced, while the rate of sperm deformation was significantly increased (P<0.05 or P<0.01), but there were no significant changes in MIXPs+ZnSO4 group. Conclusion: Zinc can inhibit the male reproductive toxicity induced by MIXPs exposure, which may be related to the improvement of the antioxidant level of testicular tissue and the increase of serum testosterone level.

Key words： zinc phthalates male reproductive toxicity antioxidant mice

收稿日期: 2018-11-28

基金资助:浙江省一流学科“公共卫生与预防医学”第一批学生创新计划项目；温州医科大学科研发展基金项目（89218006）；温州市科技局基础性科研项目（Y20180200）。

通讯作者: 高海涛，博士，讲师，Email：gaohaitaosky@163.com。

作者简介: 孙悦（1996-），女，辽宁辽阳人，本科生。

链接本文:

https://xb.wmu.edu.cn/CN/ 或 https://xb.wmu.edu.cn/CN/Y2019/V49/I9/649

[1] 中华人民共和国国家卫生和计划生育委员会. 《食品安全国家标准食品接触材料及制品用添加剂使用标准》(GB9685-2016)[S]. 北京: 2016.
[2] 高海涛, 李瑞仙, 邸倩南, 等. 我国人群邻苯二甲酸酯类的暴露水平及风险[J]. 癌变·畸变·突变, 2017, 29(6): 471-475.
[3] National Nanotechnology Coordination Office. Quantifying Exposure to Engineered Nanomaterials (QEEN) from Manufactured Products[EB/OL]. (2015-07-07)[2018-11-28].
https://www.cpsc.gov/en/Regulations-Laws--Standards/Statutes/The-Consumer-Product-Safety-Improvement-Act/Phthalates/Chronic-Hazard-Advisory-Panel-CHAP-on-Phthalates.
[4] WANG Y X, ZENG Q, SUN Y, et al. Phthalate exposure in association with serum hormone levels, sperm DNA damage and spermatozoa apoptosis: A cross-sectional study in China [J]. Environ Res, 2015, 150: 557-565.
[5] GAO H T, XU R, CAO W X, et al. Effects of six priority controlled phthalate esters with long-term low dose integrated exposure on male reproductive toxicity in rats[J]. Food Chem Toxicol, 2017, 101: 94-104.
[6] GAO H T, XU R, CAO W X, et al. Combined effects of simultaneous exposure to six phthalates and emulsifier glycerol monosterate on male reproductive system in rats[J]. Toxicol Appl Pharmacol, 2018, 341: 87-97.
[7] 王丽霞, 王雪楠. 男性不育症患者微量元素含量与精液质量关系的研究[J]. 中国医药科学, 2011, 1(16): 14-15.
[8] 梁妍琦, 钱之玉. 缺锌对雄性大鼠生殖系统的影响[J]. 中国药科大学学报, 1999, 30(1): 47-50.
[9] 李艳, 朱静媛, 庄东刚, 等. 锌对氟致雄性大鼠生殖损伤的拮抗作用[J]. 郑州大学学报(医学版), 2007, 42(1): 75-78.
[10] RAWI S M, SEIF AL NASSR F M. Zinc sulphate and vitamin E alleviate reproductive toxicity caused by aluminium sulphate in male albino rats[J]. Toxicol Ind Health, 2015, 31(3): 221-234.
[11] 刘毓谷. 卫生毒理学基础[M]. 北京: 人民卫生出版社, 1994, 215-217.
[12] ZHANG R, ZHANG L, JIANG D, et al. Mouse organ coefficient and abnormal sperm rate analysis with exposure to tap water and source water in Nanjing reach of Yangtze River[J]. Ecotoxicology, 2014, 23(4): 641-646.
[13] CHEMEK M, VENDITTI M, BOUGHAMOURA S, et al. Involvement of testicular DAAM1 expression in zinc protection against cadmium-induced male rat reproductive tox-icity[J]. J Cell Physiol, 2018, 233(1): 630-640.
[14] NORIEGA N C, HOWDESHELL K L, FURR J, et al. Pubertal administration of DEHP delays puberty, suppresses testosterone production, and inhibits reproductive tract development in male Sprague-Dawley and Long-Evans rats[J]. Toxicol Sci, 2009, 111(1): 163-178.
[15] HANNAS B R, LAMBRIGHT C S, FURR J, et al. Dose-response assessment of fetal testosterone production and gene expression levels in rat testes following in utero exposure to diethylhexyl phthalate, diisobutyl phthalate, diisoheptyl phthalate, and diisononyl phthalate[J]. Toxicol Sci, 2011, 123(1): 206-216.
[16] MOTOHASHI M, WEMPE M F, MUTOU T, et al. Male rats exposed in utero to di(n-butyl) phthalate: Age-related changes in Leydig cell smooth endoplasmic reticulum and testicular testosterone-biosynthesis enzymes/proteins[J]. Reprod Toxicol, 2016, 59: 139-146.
[17] LEHMANN K P, PHILLIPS S, SAR M, et al. Dose-dependent alterations in gene expression and testosterone synthesis in the fetal testes of male rats exposed to di (n-butyl) phthalate[J]. Toxicol Sci, 2004, 81(1): 60-68.
[18] ZHOU D, WANG H, ZHANG J. Di-n-butyl phthalate (DBP) exposure induces oxidative stress in epididymis of adult rats [J]. Toxicol Ind Health, 2011, 27(1): 65-71.
[19] NELLI G, PAMANJI S R. Di-n-butyl phthalate prompts interruption of spermatogenesis, steroidogenesis, and fertility associated with increased testicular oxidative stress in adult male rats[J]. Environ Sci Pollut Res Int, 2017, 24(22): 18563-18574.
[20] ZHANG L D, LI H C, CHONG T, et al. Prepubertal exposure to genistein alleviates di-(2-ethylhexyl) phthalate induced testicular oxidative stress in adult rats[J]. Biomed Res Int, 2014, 2014: 598630.