Sedative effect of Jiao-Tai-Wan with different concentrations and compositions on mice
NI Xiaofen1, Lin Sisi1, CHEN Qian2, YUAN Zhengzhong1
1.Department of Traditional Chinese Medicine, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, China; 2.Wenzhou Medical University, Wenzhou 325035, China
NI Xiaofen,Lin Sisi,CHEN Qian, et al. Sedative effect of Jiao-Tai-Wan with different concentrations and compositions on mice[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2019, 49(9): 639-643,648.
Abstract：Objective: To compare sedative effects of Jiao-Tai-Wan with separated prescriptions of Coptis and Cinnamon on mice. Methods: Jiao-Tai-Wan groups were intragastric administrated 7 days by Jiao-Tai-Wan respectively with high, medium and low dosage of 0.220, 0.110 and 0.055 g/mL. Coptis groups were respectively administrated 7 days by Coptis with high, medium and low dosage of 0.200, 0.100 and 0.050 g/mL. Cinnamon high dose group was administrated 7 days by Cinnamon with concentration of 0.030 g/mL. Positive control group was administrated by Zolpidem with concentration of 0.200 g/mL. Blank control group was injected with water. The locomotor activity meter was used to determine the total number of locomotor activities of mice within 15 min, 30 min, 1 h, 3 h, 12 h, 24 h after administration from day 1 to day 7. The number of locomotor activities of mice on day 8 without administration continued to be monitored. Results: On day 1 within 30 min, 1 h, 3 h, 12 h, 24 h after administration, the number of locomotor activities of Jiao-Tai-Wan group with high dose was statistically declined respectively compared with blank control group (P<0.05). These differences were observed again on day 3, day 5, day 7. These differences remained on day 8 without administration. On day 3, day 5, day 7 within 1 h, the number of locomotor activities of Jiao-Tai-Wan group with medium dose were statistically declined respectively compared with blank control group (P<0.05). On day 7 within 1 h, the number of locomotor activities of Jiao-Tai-Wan group with low dose was statistically declined comparing with blank control group (P<0.05). On day 3, day 5, day 7 within 15 min and 1 h, the frequency of locomotor activity of Coptis groups with high dosage and medium dosage were statistically declined respectively compared with blank control group (P<0.05). On day 7 within 3 h, 12 h and 24 h after drug administration, the frequency of locomotor activity of Coptis group with high dosage was statistically declined c respectively omparing with blank control group (P<0.05). Conclusion: Jiao-Tai-Wan can produce a sedative effect, in which Coptis plays a major role.
