The effect of curcumin analogue L6H3 on striatum monoamine neurotransmitter in the rat model of Parkinson disease
ZOU Yuchi1, CHEN Jun2, HUANG Wenting1, LIU Huan2, HU Jian2, LIU Fei2, WANG Dongxue2, ZHU Liyun2, LIN Li2
1. Department of Geriatric Medicine, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, China; 2.School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China
ZOU Yuchi,CHEN Jun,HUANG Wenting, et al. The effect of curcumin analogue L6H3 on striatum monoamine neurotransmitter in the rat model of Parkinson disease[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2019, 49(9): 625-629.
Abstract：Objective: To investigate the effect of curcumin analogue L6H3 on seven monoamine neurotransmitters and metabolite in the striatum of rats with Parkinson disease (PD) by micro-dialysis sampling coupled with high performance liquid chromatography and electrochemical detection (HPLC-ECD). Methods: PD model was established by injecting 6-OHDA into the right striatum of rats, then verified by the rotational behavior after intraperitoneal injection of apomorphine solution. Rats were randomly divided to Sham group, PD group and PD+L6H3 group. The microdialysis sampling coupled HPLC-ECD was developed and applied to detect the level of monoamine neurotransmitters and metabolite in rat striatum. Results: 6 weeks (2 weeks after administration),7 weeks (1 week after stopping administration) after model establishment, the levels of DA, DOPAC, HVA, NE, E, 5-HT, 5-HTAA in PD+L6H3 group were significantly increased compared with PD group (P<0.01). Conclusion: L6H3 has the capability to up-regulate monoamine neurotransmitters and metabolite in the striatum of PD rat model at both 2 weeks after administration and 1 week after stopping administration.
[1] AY M, LUO J, LANGLEY M, et al. Molecular mechanisms underlying protective effects of quercetin against mitochondrial dysfunction and progressive dopaminergic neurodegeneration in cell culture and MitoPark transgenic mouse models of Parkinson’s Disease[J]. J Neurochem, 2017, 141(5): 766-782.
[2] ZHANG N, YAN F, LIANG X, et al. Localized delivery of curcumin into brain with polysorbate 80-modified cerasomes by ultrasound-targeted microbubble destruction for improved Parkinson’s disease therapy[J]. Theranostics, 2018, 8(8): 2264-2277.
[3] Wu J Z, Cheng C C, Shen L L, et al. Synthetic chalcones with potent antioxidant ability on H2O2-induced apoptosis in PC12 cells[J]. Int J Mol Sci, 2014, 15(10): 18525-18539.
[4] CAI P, YE J, ZHU J, et al. Inhibition of endoplasmic reticulum stress is involved in the neuroprotective effect of bFGF in the 6-OHDA-induced Parkinson’s Disease model[J]. Aging Dis, 2016, 7(4): 336-449.
[5] FAN Z, YAO J, LI Y, et al. Anti-inflammatory and antioxidant effects of curcumin on acute lung injury in a rodent model of intestinal ischemia reperfusion by inhibiting the pathway of NF-Kb[J]. Int J Clin Exp Pathol, 2015, 8(4): 3451-3459.
[6] PRASAD S, TYAGI A K, AGGARWAL B B. Recent developments in delivery, bioavailability, absorption and metabolism of curcumin: the golden pigment from golden spice[J]. Cancer Res Treat, 2014, 46(1): 2-18.
[7] FARRAND A Q, HELKE K L, GREGORY R A, et al. Vagus nerve stimulation improves locomotion and neuronal populations in a model of Parkinson’s disease[J]. Brain Stimul, 2017, 10(6): 1045-1054.
[8] 叶素贞, 施剑, 夏誉, 等. 不同剂量MPTP所致帕金森病小鼠模型焦虑行为的评价[J]. 温州医科大学学报, 2016, 46(8): 586-589.
[9] SAMPAIO T B, SAVALL A S, GUTIERREZ M E Z, et al. Neurotrophic factors in Alzheimer's and Parkinson’s diseases: implications for pathogenesis and therapy[J]. Neural Regen Res, 2017, 12(4): 549-557.
[10] 赵晓蒙, 苏松坤. 高效液相色谱-电化学法检测蜜蜂脑部单胺类神经递质及其酸性代谢产物含量[J]. 福建农林大学学报(自然科学版), 2016, 45(6): 668-672.
[11] 檀笑昕, 张淑娟, 刘坤, 等. 高效液相色谱-电化学检测法研究大蒜素对百草枯中毒小鼠脑组织中单胺类神经递质含量的影响[J]. 分析测试学报, 2017, 36(9): 1069-1074.
[12] 林丽, 李永, 陈大茴, 等. 微渗析活体取样与液相色谱安培检测法联用测定鼠脑中黄嘌呤和次黄嘌呤[J]. 分析实验
室, 2005, 24(4): 43-45.
[13] LIN L1, QIU P, YANG L, et al. Determination of dopamine in rat striatum by microdialysis and high-performance liquid chromatography with electrochemical detection on a functionalized multi-wall carbon nanotube electrode[J]. Anal Bioanal Chem, 2006, 384(6): 1308-1313.
[14] 林丽, 杨小凤, 李永, 等. 微渗析活体取样-高效液相色谱电化学检测法测定鼠脑中的单胺类神经递质[J]. 研究简报,
2005, 33(5): 711-714.
[15] HE M, WANG C, WEI Y. Selective enrichment and determination of monoamine neurotransmitters by CU(II) immobilized magnetic solid phase extraction coupled with high-performance liquid chromatography-fluorescence detection[J]. Talanta, 2016, 147: 437-444.
[16] LI X S, LI S, KELLERMANN G. Simultaneous extraction and determination of monoamine neurotransmitters in human urine for clinical routine testing based on a dual functional solid phase extraction assisted by phenylboronic acid coupled with liquid chromatography-tandem mass spectrom-etry[J]. Anal Bioanal Chem, 2017, 409(11): 2859-2871.
