Preparation, characterization and in vitro observation of HER2 targeted polymer microbubbles as a contrast agent for molecular imaging
CHENG Yueyue1, YU Fangfang1, YANG Yan1, WU Senmin1, LI Jiaping2
1.Department of Ultrasound, the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou 325027, China; 2.School of Ophthalmology & Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou 325035, China
CHENG Yueyue,YU Fangfang,YANG Yan, et al. Preparation, characterization and in vitro observation of HER2 targeted polymer microbubbles as a contrast agent for molecular imaging[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2019, 49(8): 575-580.
Abstract：Objective: To prepare HER2 receptor targeted polymer microbubbles for contrast-ultrasound imaging and to evaluate their physicochemical properties, tumor targeting performance and ultrasonic imaging effect in vitro. Methods: HER2-targeted polymer microbubbles were synthesized by biotin-avidin method. The particle size, potential and stability were investigated. In vitro tumor targeting experiment, its targeting ability to HER2 (+) breast cancer cell BT-474 and HER2 (-) breast cancer cell MDA-MB-231 was investigated. The ultrasonic imaging ability was observed in vitro. Results: The average particle size of HER2-targeted polymer microbubbles was (1.78±0.37) μm, the average surface Zeta potential was (-37.40±5.74) mV. There was no statistical significant difference in the particle size of the targeted polymer microbubbles within 12 h (P>0.05). HER2-targeted polymer microbubbles exhibited the remarkable targeting ability to HER2 (+) tumor cells in vitro experiment. The microbubbles presented fine and uniform point-like dense hyperecho in vitro ultrasound imaging. Conclusion: HER2-targeted polymer microbubbles have good stability, strong targeting ability and satisfactory effect in vitro imaging, which lays a good foundation for further study of tumor targeted ultrasound imagine in vivo.
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