长链非编码RNA LINC00152在食管鳞癌组织中的表达及临床意义

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摘要目的：探索长链非编码RNA（lncRNA）LINC00152在食管鳞癌组织中的表达及临床意义。方法：选取2008年1月至2013年12月在温州医科大学附属第一医院行手术切除并经病理科确认的95例食管鳞癌患者。采用lncRNA microarray基因芯片技术，对食管鳞癌患者转移组和非转移组间癌组织和对应的癌旁正常组织lncRNA的相对表达水平进行检测，寻找差异表达的lncRNA；应用qRT-PCR技术对差异表达的lncRNA在鳞癌组织样本中进行验证；分析目标lncRNA表达水平与临床特征的相关性；绘制ROC曲线评价LINC00152作为食管鳞癌转移预测因子的效能；检索国际基因芯片lncRNA数据库相关数据，分析LINC00152表达水平和生存期的关系。结果：LncRNA microarray基因芯片实验发现食管鳞癌转移组和非转移组间lncRNA相对表达量5倍以上差异表达的共有10条；qRT-PCR验证实验发现3条lncRNA（MIR205HG、LINC00152和FOXD2-AS1）的表达量在组间差异有统计学意义（P＜0.05）；统计分析发现LINC00152的相对表达量和食管鳞癌的淋巴结转移具有相关性（P＜0.01）；ROC曲线分析显示LINC00152判断食管鳞癌转移的AUC为0.781（95%CI=0.512～0.824，P=0.032）；国际基因芯片食管癌组织数据库检索结果显示，LINC00152的高表达预示较差的生存期。结论：LINC00152可能参与了食管鳞癌的转移发生过程，是食管鳞癌预后判断新的生物标志物。

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	杨毅
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	单云峰

关键词 ：长链非编码RNA, LINC00152, 食管肿瘤, 转移

Abstract：Objective: To explore the expression and clinical significance of long non-coding RNA (lncRNA) LINC00152 in esophageal squamous cell carcinoma. Methods: A total of 95 esophageal squamous cell carcinoma patients from the First Affiliated Hospital of Wenzhou Medical University during January 2008 to December 2013. LncRNA microarray gene chip technology was used to detect the expression level of lncRNA in the cancer tissue of esophageal squamous cell carcinoma and corresponding paracancerous normal tissues between metastatic group and non-metastatic group, in order to find the differential expression of lncRNAs. The differential expression of lncRNA in squamous cell carcinoma was verified by qRT-PCR. The correlation between lncRNA expression level and clinical features was analyzed. The ROC curve was drawn to evaluate the efficacy of LINC00152 as a predictor of esophageal squamous cell carcinoma metastasis. Correlation data of international gene chip lncRNA database of esophageal squamous cell carcinoma were retrieved to analyze the relationship between LINC00152 expression level and survival. Results: LncRNA microarray gene chip experiments showed that there were 10 lncRNAs in esophageal squamous cell carcinoma and paracancerous normal tissue with more than 5 times differential expression. The qRT-PCR validation of esophageal squamous cell carcinoma and paracancerous normal tissue found that the expression of 3 lncRNAs (MIR205HG, LINC00152, FOXD2-AS1) were statistically different between metastatic group and non-metastatic group. Statistical analysis revealed that the expression of LINC00152 was correlated with the lymph node metastasis of esophageal squamous cell carcinoma (P<0.01). ROC curve analysis showed that the AUC of ROC was 0.781(95%CI=0.512-0.824, P=0.032). International gene chip database data of esophageal cancer showed that the high expression of LINC00152 predicted a poor survival. Conclusion: LINC00152 may be involved in the metastasis process of esophageal squamous cell carcinoma and is a new biomarker for the prognosis of esophageal squamous cell carcinoma.

Key words： long non-coding RNA LINC00152 esophageal neoplasms metastasis

收稿日期: 2018-10-08

基金资助:浙江省自然科学基金资助项目（LY15H160062）；温州市科技计划项目（Y20150125）。

通讯作者: 单云峰，主任医师，Email：shanyf@yahoo.com。

作者简介: 杨毅（1974-），女，浙江温州人，实验师。

链接本文:

https://xb.wmu.edu.cn/CN/ 或 https://xb.wmu.edu.cn/CN/Y2019/V49/I6/401

[1] ENZINGER P C, MAYER R J. Esophageal cancer[J]. N Engl J Med, 2003, 349(23): 2241-2252.
[2] FERLAY J, SHIN H R, BRAY F, et al. Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008[J]. Int J Cancer, 2010, 127(12): 2893-2917.
[3] SIEGEL R, NAISHADHAM D, JEMAL A. Cancer statistics, 2013[J]. CA Cancer J Clin, 2013, 63(1): 11-30.
[4] 张思维, 张敏, 李光琳, 等. 2003~2007年中国食管癌发病与死亡分析[J]. 中国肿瘤杂志, 2012, 4(21): 241-247.
[5] JEMAL A, BRAY F, CENTER M M, et al. Global cancer statistics[J]. CA Cancer J Clin, 2011, 61(2): 69-90.
[6] SVOBODA M, SLYSKOVA J, SCHNEIDEROVA M, et al.
HOTAIR long non-coding RNA is a negative prognostic factor not only in primary tumors, but also in the blood of colorectal cancer patients[J]. Carcinogenesis, 2014, 35(7): 1510-1515.
[7] GUTTMAN M, DONAGHEY J, CAREY B W, et al. lincRNAs act in the circuitry controlling pluripotency and dif-ferentiation[J]. Nature, 2011, 477(7364): 295-300.
[8] KHORSHIDI A, DHALIWAL P, YANG B B. Noncoding RNAs in tumor angiogenesis[J]. Adv Exp Med Biol, 2016, 927: 217-241.
[9] 严德文, 胡盈盈, 梁宗文, 等. 长链非编码RNA CasC7对宫颈癌细胞增殖的影响[J]. 温州医科大学学报, 2018, 48(2): 91-95.
[10] 孙祥威, 胡盈盈, 徐剑峰, 等. 长链非编码RNA AL049452在结直肠癌组织中的表达及临床意义[J]. 温州医科大学学报, 2017, 47(8): 566-570.
[11] SHEN W J, ZHANG F, ZHAO X, et al. LncRNAs and esophageal squamous cell carcinoma-implications for pathogenesis and drug development[J]. J Cancer, 2016, 7(10): 1258-1264.
[12] LI J, CHEN Z, TIAN L, et al. LncRNA profile study reveals a three-lncRNA signature associated with the survival of patients with oesophageal squamous cell carcinoma[J]. Gut, 2014, 63(11): 1700-1710.
[13] NEUMANN O, KESSELMEIER M, GEFFERS R, et al. Methylome analysis and integrative profiling of human HCCs identify novel protumorigenic factors[J]. Hepatology, 2012, 56(5): 1817-1827.
[14] LIU L, WEN J, GU X, et al. Prognostic role of long non-coding RNA LINC00152 in Chinese cancer patients: a meta-analysis[J]. Oncotarget, 2017, 8(54): 93227-93235.
[15] YANG T, ZENG H, CHEN W, et al. Helicobacter pylori infection, H19 and LINC00152 expression in serum and risk of gastric cancer in a Chinese population[J]. Cancer Epidemiol, 2016, 44: 147-153.
[16] YUE B, CAI D, LIU C, et al. Linc00152 functions as a competing endogenous RNA to confer oxaliplatin resistance and holds prognostic values in colon cancer[J]. Mol Ther, 2016, 24(12): 2064-2077.
[17] CHEN W M, HUANG M D, SUN D P, et al. Long intergenic non-coding RNA 00152 promotes tumor cell cycle progression by binding to EZH2 and repressing p15 and p21 in gastric cancer[J]. Oncotarget, 2016, 7(9): 9773-9787.
[18] HU H B, JIE H Y, ZHENG X X. Three circulating LncRNA predict early progress of esophageal squamous cell carcino-ma[J]. Cell Physiol Biochem, 2016, 40(1-2): 117-125.
[19] WU Y, TAN C, WENG W W, et al. Long non-coding RNA Linc00152 is a positive prognostic factor for and demonstrates malignant biological behavior in clear cell renal cell carcinoma[J]. Am J Cancer Res, 2016, 6(2): 285-299.
[20] PANG Q, GE J, SHAO Y, et al. Increased expression of long intergenic non-coding RNA LINC00152 in gastric cancer and its clinical significance[J]. Tumour Biol, 2014, 35(6): 5441-5447.