The effect of protectin DX on inflammation and fibroproliferation in primary lung fibroblasts
ZHENG Shengxing, PAN Jingyi, GU Lijun, YANG Jingxiang, CHEN Xinou, LI Ming, JIN Shengwei
Department of Anesthesiology and Perioperative Medicine, the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou 325027, China
ZHENG Shengxing,PAN Jingyi,GU Lijun, et al. The effect of protectin DX on inflammation and fibroproliferation in primary lung fibroblasts[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2019, 49(6): 391-396.
Abstract：Objective: To explore effects of protectin DX (PDX) on the expression of cyclooxygenase-2 (COX-2), fibroproliferation, collagen production and myofibroblast differentiation in mice primary lung fibroblast cells (LF). Methods: After the lung fibroblasts were isolated and purified, the acute inflammation model and the fibriproliferation model was respectively established with lipopolysaccharide (LPS) and with TGF-β1. Then primary lung fibroblast cells were incubated in DMEM medium containing LPS or TGF-β1 in the presence or absence of PDX. After incubation, cell viability was measured using cell titer. mRNA expression was measured by realtime-PCR. The cells were harvested, and COX-2 protein was analyzed by western-blot. Supernatants from lung fibroblast cells were collected and PGE2 protein expression was measured by ELISA. Results: It is a reasonable model of fibroproliferation in fibroblasts by administering 10 ng/mL TGF-β1 for 48 hours. PDX reduced LPS-induced expression of COX-2 and production of PGE2, while inhibiting TGF-β1-induced lung fibroblast cells proliferation in a concentration dependent manner. In addition, PDX reduced rat primary LF collagen production, α-smooth muscle actin (α-SMA) and fibronectin expression induced by TGF-β1. Conclusion: PDX inhibits LPS-induced expression of COX-2 and production of PGE2 in primary lung fibroblast cells, while decreasing TGF-β1-induced fibroproliferation, collagen production and differentiation in primary lung fibroblast cells.
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