去甲亚精胺对白色念珠菌生物膜形成的抑制作用

摘要
图/表
参考文献
相关文章 (6)

全文: PDF (1470 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要目的：研究去甲亚精胺对白色念珠菌生物膜形成的作用及可能的机制。方法：通过结晶紫染色、甲基四氮盐（XTT）检测、菌落形成单位（CFU）计数、扫描电镜（SEM）成像和qRT-PCR分别检测或观察去甲亚精胺对白色念珠菌生物膜生物量、代谢活性、菌落数、真菌形态和毒力相关基因表达的影响。结果：结晶紫染色结果显示不同浓度去甲亚精胺对白色念珠菌生物膜形成的抑制作用不同；XTT检测结果表明去甲亚精胺能显著抑制白色念珠菌生物膜的代谢活性；CFU计数结果显示去甲亚精胺能显著减少生物膜中白色念珠菌的菌落数；SEM结果显示去甲亚精胺能显著抑制生物膜形成，高浓度的去甲亚精胺能促进酵母相形成；qRT-PCR结果显示去甲亚精胺能显著抑制白色念珠菌毒力相关基因hwp、als3、csh1的表达。结论：去甲亚精胺能显著抑制白色念珠菌生物膜形成的作用并抑制毒力基因的表达，是一种潜在的抑制白色念珠菌生物膜的多胺类药物。

	服务
	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	曹焱帆
	章可可
	张燕妮
	汤丰瑜
	胡凤婷
	叶青松

关键词 ：去甲亚精胺, 白色念珠菌, 生物膜, 基因表达

Abstract：Objective: To study the effect of norspermidine on Candida albicans (C.albicans) biofilm formation and the underlying mechanisms. Methods: Crystal violet staining, XTT assay, colony-forming unit (CFU) count, scanning electron microscope (SEM) imaging and qRT-PCR was conducted to detect the effect of norspermidine on biofilm biomass, metabolic activity, colony number, fungal morphology and virulence-related gene expression of C.albicans biofilm. Results: Crystal violet staining results demonstrated that different concentrations of norspermidine had different inhibitory effects on C.albicans biofilm formation. XTT assay showed that norspermidine significantly restrained the metabolic activity of C.albicans biofilm. CFU counting results indicated that norspermidine severely reduced the number of C.albicans colonies in biofilm. SEM imaging displayed that norspermidine dramatically disturbed the formation of C.albicans biofilm and norspermidine in high concentration promoted the occurrence of yeast phase; qRT-PCR showed that norspermidine substantially interfered with the gene expression of ywp, hwp and als3, which were related to the virulence of C.albicans. Conclusion: Norspermidine could be used as a potential polyamine drug for anti- C.albicans biofilm as it could not only inhibit the formation of C.albicans biofilm but also interfere with the expression of its virulence-associated gene expression.

Key words： norspermidine candida albicans biofilm gene expression

收稿日期: 2019-02-10

基金资助:国家自然科学基金资助项目（81700960）；浙江省公益技术应用社会发展项目（LGF19H140004）；温州市科技计划项目（Y20180163）。

通讯作者: 叶青松，教授，硕士生导师，Email：qingsongye@hotmail.com。

作者简介: 曹焱帆（1991-），男，浙江温州人，硕士生。

链接本文:

https://xb.wmu.edu.cn/CN/ 或 https://xb.wmu.edu.cn/CN/Y2019/V49/I5/321

[1] ROY R, TIWARI M, DONELLI G, et al. Strategies for combating bacterial biofilms: A focus on anti-biofilm agents and their mechanisms of action[J]. Virulence, 2018, 9(1): 522-554.
[2] SZTAJER H, SZAFRANSKI S P, TOMASCH J, et al. Cross-feeding and interkingdom communication in dual-species biofilms of Streptococcus mutans and Candida albi-cans[J]. ISME J, 2014, 8(11): 2256-2271.
[3] PENESYAN A, GILLINGS M, PAULSEN I T. Antibiotic discovery: combatting bacterial resistance in cells and in biofilm communities[J]. Molecules, 2015, 20(4): 5286-5598.
[4] DO D C. The role of bacterial biofilms in chronic infections [J]. APMIS Suppl, 2013(136): 1-51.
[5] FLAMIGNI F F, ROSSONI C, STEFANELLI C, et al. Polyamine metabolism and function in the heart[J]. J Mol Cell Cardiol, 1986, 18(1): 3-11.
[6] KARATAN E, DUNCAN T R, WATNICK P I. Nsps. a predicted polyamine sensor, mediates activation of vibrio Cholerae biofilm formation by norspermidine[J]. J Bacteriol, 2005, 187(21): 7434-7443.
[7] FELDMAN M, SHENDEROVICH J, Al-QUNTAR A A, et al. Sustained release of a novel anti-Quorum-sensing agent against oral fungal biofilms[J]. Antimicrob Agents Chemother, 2015, 59(4): 2265-2272.
[8] UPPULURI P, SRINIVASAN A, RAMASUBRAMANIAN A, et al. Effects of Fluconazole, Amphotericin B, and Caspofungin on Candida albicans biofilms under conditions of flow and on biofilm dispersion[J]. Antimicrob Agents Chemother, 2011, 55(7): 3591-3593.
[9] HSU C C, LAI W L, CHUANG K C, et al. The inhibitory activity of Linalool against the filamentous growth and biofilm formation in Candida albicans[J]. Med Mycol, 2013, 51(5): 473-482.
[10] OU M, LING J. Norspermidine changes the basic structure of S. mutans biofilm[J]. Mol Med Rep, 2017, 15(1): 210-220.
[11] RAMAGE G, SAVILLE S P, WICKES B L, et al. Inhibition of Candida albicans biofilm formation by Farnesol, a Quorum-sensing molecule[J]. Appl Environ Microbiol, 2002, 68(11): 5459-5463.
[12] SENEVIRATNE C J, SILVA W J, JIN L J, et al. Architectural analysis, viability assessment and growth kinetics of Candida albicans and Candida glabrata biofilms[J]. Arch Oral Biol, 2009, 54(11): 1052-1060.
[13] SAMARANAYAKE Y H, CHEUNG B P, YAU J Y, et al. Human serum promotes Candida albicans biofilm growth and virulence gene expression on silicone biomaterial[J]. PLoS One, 2013, 8(5): e62902.
[14] LI D D, ZHAO L X, MYLONAKIS E, et al. In vitro and in vivo activities of pterostilbene against Candida albicans bio-films[J]. Antimicrob Agents Chemother, 2014, 58(4): 2344-2355.
[15] CAVALCANTI Y W, MORSE D J, DA SILVA W J, et al. Virulence and Pathogenicity of Candida albicans is enhanced in biofilms containing oral bacteria[J]. Biofouling, 2015, 31(1): 27-38.
[16] 王文花, 李劲松, 胡臻, 等. 黄连对系统性白色念珠菌病小鼠模型影响的实验研究[J]. 温州医学院学报, 2013, 43(2): 112-114.
[17] NESSE L L, BERG K, VESTBY L K. Effects of norspermidine and spermidine on biofilm formation by potentially pathogenic Escherichia coli and Salmonella enterica wild-type strains[J]. Appl Environ Microbiol, 2015, 81(6): 2226-2232.
[18] da Silva Dantas A, Lee K K, Raziunaite I, et al. Cell biology of Candida albicans-host interactions[J]. Curr Opin Microbiol, 2016, 34: 111-118.
[19] HOBLEY L, KIM S H, MAEZATO Y, et al. Norspermidine is not a self-produced trigger for biofilm disassembly[J]. Cell, 2014, 156(4): 844-854.
[20] METWALLI K H, KHAN S A, KROM B P, et al. Streptococcus mutans, Candida albicans, and the human mouth: a sticky situation[J]. PLoS Pathogens, 2013, 9(10): e1003616.
[21] MAYER F L, WILSON D, HUBE B. Candida albicans pathogenicity mechanisms[J]. Virulence, 2013, 4(2): 119-128.
[22] MARK F, ISAAC G, ABED A Q, et al. Thiazolidinedione-8 alters symbiotic relationship in C. albicans-S. mutans dual species biofilm[J]. Front Microbiol, 2016, 7: 140.