琼脂糖/ε-PLL/CHA屏障膜的制备及其抑菌能力和生物性能评价

摘要
图/表
参考文献
相关文章 (7)

全文: PDF (1506 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要目的：制备琼脂糖/ε-聚赖氨酸（ε-PLL）/碳酸羟基磷灰石（CHA）新型牙周引导组织再生（GTR）屏障膜，并评价其生物相容性和抗菌性能。方法：首先采用水热法制备CHA，通过扫描电镜（SEM）和傅里叶红外光谱（FTIR）分析CHA的形态和构象；采用琼脂糖水凝胶包载CHA和ε-PLL制备屏障膜；流变仪检测屏障膜的机械性能；活细胞/死细胞（Live/Dead）染色检测生物相容性；抑菌环实验证实对金黄色葡萄球菌的抗菌性能。结果：SEM及FTIR分析表明成功合成中空柱状的CHA。CHA浓度的增加，显著增强其机械性能；Live/Dead染色结果显示屏障膜具有良好的生物相容性；在屏障膜周围可见明显的抑菌环。结论：成功制备琼脂糖/ε-PLL/CHA屏障膜，并证明其具有良好的生物相容性及抗菌性能。

	服务
	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	何智琪
	钱秋萍
	邓辉
	胡荣党

关键词 ：屏障膜, 琼脂糖, 聚赖氨酸, 碳酸羟基磷灰石, 抗菌

Abstract：Objective: To prepare a novel periodontal guided tissue regeneration (GTR) barrier membranes of agarose/ε-PLL/CHA and evaluation of it with excellent biocompatibility and antibacterial properties. Methods: CHA was prepared by the hydrothermal method. The characterization of the CHA were analyzed by Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). The different concentrations of CHA and ε-PLL were added to the agarose to prepare the barrier membranes. The mechanical strength of barrier membranes was assessed through rheometer. Live/Dead staining was used to detect the biocompatibility of barrier membranes. The effect of antibacterial of barrier membranes was confirmed by inhibition zone. Results: SEM and FTIR indicated successful synthesis of hollow columnar CHA. Barrier membranes have good biocompatibility. CHA could enhance the mechanical properties of the barrier membranes. Live/Dead staining showed a good biocompatibility. There were obvious inhibition zones found around the barrier membranes. Conclusion: The agarose/ε-PLL/CHA barrier membrane was successfully prepared, and the membrane was proved to have good biocompatibility and antibacterial properties.

Key words： barrier membrane agarose poly-L-lysine carbonated hydroxyapatite antibacterial

收稿日期: 2018-12-17

基金资助:浙江省公益技术应用研究项目（LGF18H140007，LGF 19H140001）。

通讯作者: 胡荣党，教授，硕士生导师，Email：hurongdang@hotmail.com。

作者简介: 何智琪（1993-），女，浙江瑞安人，硕士生。

链接本文:

https://xb.wmu.edu.cn/CN/ 或 https://xb.wmu.edu.cn/CN/Y2019/V49/I4/267

[1] ZHANG E, ZHU C, YANG J, et al. Electrospun PDLLA/PLGA composite membranes for potential application in guided tissue regeneration[J]. Mater Sci Eng C Mater Biol Appl, 2016, 58: 278-285.
[2] SOLTANI DEHNAVI S, MEHDIKHANI M, RAFIENIA M,
et al. Preparation and in vitro evaluation of polycaprolactone/PEG/bioactive glass nanopowders nanocomposite membranes for GTR/GBR applications[J]. Mater Sci Eng C Mater Biol Appl, 2018, 90: 236-247.
[3] VERÍSSIMO D M, LEITÃO R F, FIGUEIRÓ S D, et al. Guided bone regeneration produced by new mineralized and reticulated collagen membranes in critical-sized rat calvarial defects[J]. Exp Biol Med (Maywood), 2015, 240(2): 175-184.
[4] DEYMIER A C, NAIR A K, DEPALLE B, et al. Protein-free formation of bone-like apatite: New insights into the key role of carbonation[J]. Biomaterials, 2017, 127: 75-88.
[5] GUO Y J, LONG T, CHEN W, et al. Bactericidal property and biocompatibility of gentamicin-loaded mesoporous carbonated hydroxyapatite microspheres[J]. Mater Sci Eng C Mater Biol Appl, 2013, 33(7): 3583-3591.
[6] YAO Q, YE Z, SUN L, et al. Bacterial infection microenvironment-responsive enzymatically degradable multilayer films for multifunctional antibacterial properties[J]. J Mater Chem B, 2017, 5(43): 8532-8541.
[7] ZHANG L, LI R, DONG F, et al. Physical, mechanical and antimicrobial properties of starch films incorporated with ε-poly-L-lysine[J]. Food Chem, 2015, 166: 107-114.
[8] NYMAN S, LINDHE J, KARRING T, et al. New attachment following surgical treatment of human periodontal dis-ease[J]. J Clin Periodontol, 1982, 9(4): 290-296.
[9] XUE J, HE M, NIU Y, et al. Preparation and in vivo efficient anti-infection property of GTR/GBR implant made by metronidazole loaded electrospun polycaprolactone nanofiber membrane[J]. Int J Pharm, 2014, 475(1-2): 566-577.
[10] LIAO S, WANG W, UO M, et al. A three-layered nano-carbonated hydroxyapatite/collagen/PLGA composite membrane for guided tissue regeneration[J]. Biomaterials, 2005, 26(36): 7564-7571.
[11] SUZAWA Y, KUBO N, IWAI S, et al. Biomineral/agarose composite gels enhance proliferation of mesenchymal stem cells with osteogenic capability[J]. Int J Mol Sci, 2015, 16(6): 14245-14258.
[12] NASAJPOUR A, ANSARI S, RINOLDI C, et al. A multifunctional polymeric periodontal membrane with osteogenic and antibacterial characteristics[J]. Adv Funct Mater, 2018, 28(3): 1703437.
[13] LV Q, NAIR L, LAURENCIN C T. Fabrication, characterization, and in vitro evaluation of poly (lactic acid glycolic acid)/nano-hydroxyapatite composite microsphere-based scaffolds for bone tissue engineering in rotating bioreactors [J]. J Biomed Mater Res A, 2009, 91(3): 679-691.
[14] NONOYAMA T, WADA S, KIYAMA R, et al. Double-Network Hydrogels Strongly Bondable to Bones by Spontaneous Osteogenesis Penetration[J]. Adv Mater, 2016, 28(31): 6740-6745.
[15] 范子梁, 金冰慧, 徐霞芳, 等. 包载姜黄素纳米胶束的制备与体外抗肿瘤评价[J]. 温州医科大学学报, 2017, 47(9): 625-630, 636.
[16] AMARIEI G, KOKOL V, VIVOD V, et al. Biocompatible antimicrobial electrospun nanofibers functionalized with ε-poly-l-lysine[J]. Int J Pharm, 2018, 553(1-2): 141-148.
[17] YOSHIDA T, NAGASAWA T. Epsilon-Poly-L-lysine: microbial production, biodegradation and application potential[J]. Appl Microbiol Biotechnol, 2003, 62(1): 21-26.
[18] 张敏, 高家诚, 王勇, 等. HA生物陶瓷的掺杂改性研究进展[J]. 材料导报, 2005, 19(2): 20-22.
[19] LI B, LIAO X, ZHENG L, et al. Preparation and cellular response of porous A-type carbonated hydroxyapatite nanoc-eramics[J]. Mat Sci Eng C, 2012, 32(4): 929-936.
[20] HU H, QIAO Y, MENG F, et al. Enhanced apatite-forming ability and cytocompatibility of porous and nanostructured TiO2/CaSiO3 coating on titanium[J]. Colloids Surf B Biointerfaces, 2013, 101: 83-90.
[21] MORGAN E F, YETKINLER D N, CONSTANTZ B R, et al.
Mechanical properties of carbonated apatite bone mineral substitute: strength, fracture and fatigue behaviour[J]. J Mater Sci Mater Med, 1997, 8(9): 559-570.