GUAN Jiaqing,XU Jiadong,WANG Chunyong, et al. Roles of microRNA-17-5p in the resistance of MMQ cells to Cabergoline[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2019, 49(4): 258-262,280.
Abstract：Objective: To study the roles of microRNA-17-5p may play in the resistance of MMQ cells to dopamine agonists. Methods: MMQ cells were either treated with miR-17-5p mimics or miR-17-5p inhibitor to regulate the expression of miR-17-5p in vitro qPCR was used to verify the transfection efficiency. After treatment, cell viability and its response to CAB was determined with CCK-8 assays. The target gene of miR-17-5p was predicted and confirmed via bioinformatics analysis and luciferase reporter assays. The expression of PTEN was analyzed by qPCR and Western Blotting. In addition we deployed CCK-8 assay to evaluate the response to CAB of MMQ cells which were initially treated with lentiviral vector containing PTEN. Results: Overexpression of miR-17-5p could promote the proliferation of MMQ cells and suppress CAB cytotoxicity. On the contrary, down-regulated of the expression of miR-17-5p could effectively inhibit proliferation and boost susceptibility to CAB in MMQ cells. The bioinformatics database identified the potential target of miR-17-5p and the luciferase reporter assay confirmed that PTEN was the direct target of miR-17-5p. While the expression of PTEN was down-regulated in miR-17-5p over-expressed MMQ cells, the expression of PTEN was up-regulated in miR-17-5p knockdown MMQ cells. Furthermore, PTEN over-expression was able to reverse the drug resistance induced by miR-17-5p on CAB treatment. Conclusion: miR-17-5p promotes MMQ proliferation and mediates CAB resistance by targeting PTEN.
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