Conservative analysis in CTL epitopes of HBV S protein between B and C genotypes
CHEN Qingxin1, LI Wenshu1, Chen Zhuoyan1, HE Jiali1, Wang Wenhuan1, Lin Mao1, YE Juxiu2
1.Institute of Microbiology and Immunology, Wenzhou Medical University, Wenzhou 325035, China; 2.School of Ophthalmology & Optometry, Wenzhou Medical University, Wenzhou 325035, China
CHEN Qingxin,LI Wenshu,Chen Zhuoyan, et al. Conservative analysis in CTL epitopes of HBV S protein between B and C genotypes[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2019, 49(4): 253-257.
Abstract：Objective: To analyze the conservative property in CTL epitopes of HBV large S proteins and figure out the stable epitopes between B and C genotypes. Methods: A pair of primers for S gene of HBV was designed, the S gene amplified by PCR and construed into pIRES vector. According to large S protein from B and C genotypes of HBV, specific CTL epitopes in large S protein of HBV had been analyzed and screened by BIMAS, NetMHC and SYFPEITHI. Conservative property analysis was made by comparing candidate CTL epitopes with large S protein database of HBV B and C genotypes conducted by BLAST. Results: HBV S gene was cloned into pIRES expression vector successfully. Seventeen CTL epitopes of large S protein were screened according to scores of BIMAS, NetMHC or SYFPEITHI. High conservative property in B and C genotypes of HBV (>80%) was showed in 6 CTL epitopes which were not identified now. 17 CTL epitopes, including 6 identified CTL epitopes, were not conservative in B and C genotypes of HBV. Conclusion: The specific CTL epitopes of large S protein of HBV show high differences between B and C genotypes. Six CTL epitopes of HBV large S protein are obtained for further immunological studies.
