ZHOU Linjie,ZHENG Zhencang,WANG Xin, et al. HLA-G expression and its clinical significance by HBV[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2014, 44(6): 435-.
Abstract：Objective: To investigate HLA-G expression and its clinical significance by HBV. Methods: Real-time RT-PCR and enzyme-linked immunosorbent assay (ELISA) were used to explore the serum HBV DNA load and plasma sHLA-G concentrations. HBV and THP-1 were cocultured for 1~6 d. RT-PCR and Western blot were used to analyse the expression of HLA-G in THP-1. Results: Plasma sHLA-G levels were dramatically increased in the peripheral blood of patients with HBV when compared with that in normal controls (P<0.01). After in vitro infection by a highly pathogenic strain of HBV, HLA-G mRNA was dramatically increased and the protein expression of HLA-G was induced. Datas showed that HLA-G transcription and protein expression were induced in 1 day and reached highest on 4 day and then decreased in a manner of time-dependent. Conclusion: Given its immunotolerant property, an increase in HLA-G of patients with HBV may play an important role in regulation of the inflammatory response process in vivo.
