LI Junjian,CHEN Gang,ZHU Qiandong, et al. Inhibition of apoptosis in pancreatic cancer cells by miR-181a targeting CARF gene[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2019, 49(3): 167-171.
Abstract：Objective: To explore the possible relationship between miR-181a and its predicted target gene-CARF, and to investigate their roles in regulating proliferation/apoptosis of pancreatic carcinoma cells. Methods: The miR-181a expression of pancreatic tissues/cells was detected by real-time fluorescent quantitative PCR. The miR-181a level was down-regulated by miR-181a inhibitors, and the proliferation activity of pancreatic cancer cells was examined by MTT. A PGL-3 vector containing the 3’-UTR sequence of the CARF gene was established to observe the relationship of miR-181a and CARF revealed by Dual Luciferase Reporter Assay System. The miR-181a level was regulated by agonists and inhibitors. Western blot was used to detect the expression of CARF protein. Results: Compared with normal pancreatic tissues and cells, miR-181a expression of pancreatic cancer tissues/cells was significantly increased (P<0.05). MiR-181a inhibitors significantly reduced the level of miR-181a and decreased its proliferative activity obviously (P<0.05). The Dual Luciferase Reporter Assay System indicated that miR-181a was co-transfected with a vector containing the 3’-UTR sequence of CARF, which significantly reduced luciferase activity (P<0.05). There was significant negative correlation between CARF protein expression and miR-181a concentration in pancreatic cancer cells (P<0.05). Conclusion: MiR-181a in pancreatic cancer tissues and cells was significantly increased, and promoted the development of pancreatic cancer through down-regulating its target gene CARF to enhance cell proliferation.
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