Exploration of the relationship between LTF gene expression and its DNA promotor region methylation in human gastric cancer cell lines BGC823
1.Department of Gastroenterology and Hepatology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015; 2.Renji College, Wenzhou Medical University, Wenzhou, 325035
ZHUGE Xiaoju1,CHEN Renpin1,HUANG Xielin2, et al. Exploration of the relationship between LTF gene expression and its DNA promotor region methylation in human gastric cancer cell lines BGC823[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2014, 44(6): 426-.
Abstract：Objective: To detect the methylation status and mRNA expression of LTF (lactotransferrin) gene in BGC823 gastric cancer cell lines, to explore the relationship between the abnormal methylation of LTF gene’s promoter region and its expression in gastric cancer. Methods: Bisulfite genomic sequencing PCR (BSP) was adopted to detect the promotor methylation status of LTF. Real-time PCR and Western blot were respectively used to evaluate LTF expression in mRNA and protein level. Meanwhile, 5-aza-CdR was used to reverse the LTF genes’ methylation status, and after the treatment, methylation and mRNA expression status was recorded. Results: The promotor methylation rates of LTF in BGC823 was 53.6%. Compared to the control group, methylation status and mRNA expression of LTF in drug treatment groups (the 5-aza-CdR concentration was 2 μmol/L and 10 μmol/L) were reversed by 5-aza-CdR, which showed statistical differences (P<0.05). But no diffenrence had shown between different treatment groups (P>0.05). Conclusion: The LTF methylation in promoter region is high in BGC823 which is related with the low expression of mRNA level. Demethylation agents can reverse the methylation status and re-express its mRNA.
