ZHU Suiyong,JIN Fangsi,SHI Shunqiu, et al. Establishment of modified microcolumn gel method for detection of ABO variant blood type[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2019, 49(2): 104-107.
Abstract：Objective: To establish a modified microcolumn gel method for the detection of ABO variant blood type. Methods: From November 2016 to May 2018, 12 patients with ABO blood group agglutination intensity change or inconsistent positive and negative stereotypes were selected from the Second Affiliated Hospital &
Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou People’s Hospital and Rui’an People’s Hospital. The ABO blood type of the specimens was confirmed by blood type serogy test and blood type gene test combined with clinical manifestations. The known blood group specimens were detected by microcolumn gel method. A modified microcolumn gel method was established by using 1% O, A3 subtype and A antigen weakened erythrocytes to adjust the way of centrifugation of microcolumn gel method, which was used to detect all the samples. Results: Of the 12 cases, there was 1 case of A3 subtype, 3 cases of B(A) type, 3 cases of Mumbai type and 5 cases of ABO blood group antigen with weak expression. The sensitivity of microcolumn gel method was similar to that of saline tube test. Only 2 of 12 specimens were detected, with the detection rate being 2/12. The centrifugation method of the modified microcolumn gel method was 900 r/min centrifugation 2 min, 30 min incubated at 4 °C and 1 800 r/min centrifugation 3 min. Only 1 case of the 12 specimens could not be detected by the modified microcolumn gel method, the detection rate being 11/12. Conclusion: The modified microcolumn gel method established in this study improves the detection sensitivity by changing the centrifuge mode and increased incubation time and incubation temperature of the microcolumn gel blood type card. It is suitable for screening ABO variant blood groups.
