ZHANG Haijuan,ZHANG Qilu,XU Ying, et al. Synthesis of zinc hyaluronate and application in wound repair in mice[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2019, 49(1): 43-46，51.
Abstract：Objective: To react sodium hyaluronate with zinc chloride, then identify the physicochemical properties of the zinc hyaluronate, and observed its effect on wound healing in mice. Methods: The rubber ring was sewn with eight stitches onto the skin of 10 mice, half male and half female, to make a full-thickness wound model of the skin on the left and right sides of the mice. The left and right side were respectively coated with zinc hyaluronate solution and sodium hyaluronate solution, wrapped in sterile gauze. The medicine was changed once a day, and the wound healing of each group was observed after the operation. The sodium ion detector was used to detect the formation of zinc hyaluronate, and then the antibacterial effect of zinc hyaluronate was studied using spread plate technique. Elemental analysis was made to detect the sodium and zinc ions content of zinc hyaluronate. Results: Sodium hyaluronate reacted with zinc chloride to form zinc hyaluronate, in which zinc ions were covalently bonded with hyaluronic acid. Zinc hyaluronic acid solution also had antibacterial function (P<0.05). The results of whole skin resection in mice showed that zinc hyaluronate was more effective than sodium hyaluronate to promote wound repair (P<0.05). Conclusion: Hyaluronic acid and zinc can be combined by covalent bond, and zinc hyaluronate solid with high purity can be obtained by dialysis. Zinc hyaluronate can promote wound healing better than sodium hyaluronate.
