Abstract：Objective: To establish obstructive sleep apnea and hypopnea syndrome (OSAHS) enuretic model induced by chronic intermittent hypoxia (CIH) exposure in female rat, and to explore the role of catechol estrogen (CE) in CIH induced OSAHS enuretic model. Methods: Sixteen Sprague-Dawley (SD) female rats (6 weeks old) were randomly divided into CIH group and the control group, with eight rats for each group. The CIH group was exposed to chronic intermittent hypoxia cabin for 4 weeks; meanwhile, the control group was exposed to normal air cabin. Water intake and urine output were recorded; the levels of CE, NA, Adr and DA in serum and the activity of COMT were measured, and threshold pressure was measured; bladder was taken for HE and Weigert staining, then the histological changes under light microscope were observed. Results: Compared with the control group, the water intake and urine output outside the cabin in CIH group had no significant difference. However, there was significant increase of urine output inside cabin in CIH group. Furthermore, the levels of CE, NA, Adr and DA in serum were significantly higher, whereas the activity of COMT and the threshold pressure were significantly decreased compared with that in the control group. HE staining showed the disordered arrangement of bladder detrusor muscle bundle and some breaking muscle bundles. Conclusion: CIH induces enuresis in female rats accompanying with increased urine output and CE, NA, Adr and DA levels in serum, decreased activity of COMT and threshold pressure, which are similar to the clinical characteristics in enuresis children. It suggests that it is feasible to use CIH to establish OSAHS enuretic female rat model.
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