Correlation of Fsp27 with hepatic fibrosis pathology and liver fibrosis index
YU Xia1, HUANG Kate1, YU Fuxiang2.
1.Department of Pathology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015; 2.Department of Hepatobiliary and Pancreatic Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015
YU Xia,HUANG Kate,YU Fuxiang.. Correlation of Fsp27 with hepatic fibrosis pathology and liver fibrosis index[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2018, 48(12): 909-912.
Abstract：Objective: To study the correlation of Fsp27 with liver fibrosis pathology and liver fibrosis index. Methods: In 45 cases of liver surgery from July 2015 to July 2017 in the First Affiliated Hospital of Wenzhou Medical University, HE staining, Fsp27 protein staining and pathological staging were performed. The expression of Fsp27 in liver was determined by immunohistochemical assay, Real-time PCR and Western blot respectively. Results: The positive expression of Fsp27 in liver tissue was mainly in primary hepatic stellate cells. Compared with normal control group, the expression of Fsp27 at mRNA and protein level was negatively correlated with the stage of hepatic fibrosis (P<0.05). There was also a negative correlation between the levels of Fsp27 and collagen III, hyaluronic acid and hydroxyproline expression (r=-0.521, -0.834, -0.667, P<0.05). Conclusion: The expression of Fsp27 is negatively correlated with the degree of hepatic fibrosis.
[1] 蔡伟祥, 方建凤, 庄伟, 等. 分离、鉴定与培养大鼠肝细胞、
肝星状细胞和Kupffer细胞[J]. 温州医科大学学报, 2016, 46(9): 638-643, 648.
[2] RAY K. Liver: hepatic stellate cells hold the key to liver fibrosis[J]. Nat Rev Gastroenterol Hepatol, 2014, 11(2): 74.
[3] 吴琳石, 邱江锋, 吴志勇. 肝纤维化时星状细胞激活的分子生物学机制[J]. 肝胆胰外科杂志, 2006, 18(1): 58-60.
[4] AIBARA D, MATSUSUE K, MATSUO K, et al. Expression of hepatic fat-specific protein 27 depends on the specific etiology of fatty liver[J]. Biol Pharm Bull, 2013, 36(11): 1766-1772.
[5] 俞富祥, 宋才鑫, 吴志伟, 等. 脂肪特异性蛋白27基因抑制大鼠肝星状细胞增殖活化[J]. 中华肝胆外科杂志, 2013, 19(9): 701-705.
[6] YU F, SU L, JI S, et al. Inhibition of hepatic stellate cell activation and liver fibrosis by fat-specific protein 27[J]. Mol Cell Biochem, 2012, 369(1-2): 35-43.
[7] 中华医学会肝病学分会、中华医学会感染病分会. 慢性乙型肝炎防治指南(2010年版)[J]. 实用肝脏病杂志, 2011, 14(2): 81-89.
[8] ATTIA Y M, ELALKAMY E F, HAMMAM O A, et al. Telmisartan, an AT1 receptor blocker and a PPAR gamma activator, alleviates liver fibrosis induced experimentally by Schistosoma mansoni infection[J]. Parasit Vectors, 2013, 6: 199.
[9] ZHOU D, WANG J, HE L N, et al. Prolyl oligopeptidase attenuates hepatic stellate cell activation through induction of Smad7 and PPAR-γ[J]. Exp Ther Med, 2017, 13(2): 780-786.
[10] GUAN W, CHENG F, WU H, et al. GATA binding protein 3 is correlated with leptin regulation of PPARγ1 in hepatic stellate cells[J]. J Cell Mol Med, 2017, 21(3): 568-578.
[11] MATSUSUE K, KUSAKABE T, NOGUCHI T, et al. Hepatic steatosis in leptin-deficient mice is promoted by the PPARgamma target gene Fsp27[J]. Cell Metab, 2008, 7(4): 302-311.
