Value of real-time myocardial contrast echocardiography in the detection of the myocardial perfusion abnormalities in early diabetic rats model
1.Department of Ultrasonography, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325027; 2.School of Pharmacy, Wenzhou Medical University, Wenzhou, 325035
ZHENG Lei1,ZHANG Ming2,LIANG Tianlin1, et al. Value of real-time myocardial contrast echocardiography in the detection of the myocardial perfusion abnormalities in early diabetic rats model[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2014, 44(6): 407-.
Abstract：Objective: To explore the value of real-time myocardial controast echocardiography to detect myocardial perfusion abnormalities in early diabetic rats. Methods: Among twenty-four male SD rats, twelve rats were randomly selected as DM group which were induced with streptozotocin, the remain twelve rats as normal control group which were induced with buffer solution. Eight weeks after diabetic model established, all rats underwent MCE examination at the resting state. The plateau intensity (A), initial slope of the curve (β) and myocardial blood flow (A×β) of left ventricular anterior wall at the papillary muscle level were measured in left ventricular short-axis view by MCE. At last, myocardial tissue of the rats were stained with CD31 immunohistochemistry to detect myocardial microvascular density (MVD). The correlation between A and MVD at DM group was analyzed. Results: Compared with the control group, A and A×β in DM group were obviously lower (P<0.01), β in DM group was also lower, but without statistical difference. The MVD in the DM group was significantly decreased compared with the control group (P<0.01). There were significant positive correlations between A and MVD at DM group (r=0.903, P<0.01). Conclusion: MCE can be used to detect the left ventricular myocardial perfusion abnormalities in early diabetic rats and has important application value in diagnosis of the diabetic cardiomyopathy.
