黄芪多糖对棕榈酸损伤的人脐静脉内皮细胞内一氧化氮水平的影响

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摘要目的：探讨黄芪多糖对棕榈酸损伤的人脐静脉内皮细胞内一氧化氮（NO）水平的影响。方法：用棕榈酸诱导人脐静脉内皮细胞建立内皮细胞损伤模型，实验设为对照组、模型组、黄芪多糖组（400 mg/L、800 mg/L、1 600 mg/L）。MTT试验检测黄芪多糖对细胞活性的影响。Western blot检测细胞内AMPK、p-AMPK、p-Akt、eNOS、p-eNOS、Nox4、GAPDH蛋白的表达。Q-PCR试验检测Nox4 mRNA以及eNOS mRNA的表达。硝酸酶还原法检测细胞内NO含量。结果：黄芪多糖可以上调棕榈酸损伤的人脐静脉内皮细胞内p-AMPK、p-Akt、p-eNOS蛋白以及eNOS mRNA的表达（P＜0.05）；下调Nox4蛋白及Nox4 mRNA的表达，增加细胞内NO的生成（P＜
0.05）。结论：黄芪多糖可以促进内皮细胞内NO的生成，其调控机制可能与其能活化细胞内AMPK、Akt蛋白，激活eNOS以及降低Nox4的表达有关。

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	胡兰兰
	陈佳君
	陈君第霞
	潘晓琼
	胡臻

关键词 ：黄芪多糖, 血管内皮功能障碍, 一氧化氮, 动脉粥样硬化

Abstract：Objective: To explore and investigate the effects of Astragalus polysaccharides on nitric oxide in human umbilical vein endothelial cells induced with palmitic acid. Methods: Endothelial cell injury model was established by using human umbilical vein endothelial cells induced with palmitic acid. The experiment consisted of control group, model group, astragalus polysaccharide group (400 mg/L, 800 mg/L, 1 600 mg/L). The effects of Astragalus polysaccharides on cell viability was tested by MTT assay. Western blotting assay was used to detect the expressions of AMPK, p-AMPK, p-Akt, eNOS, p-eNOS, Nox4, and GAPDH. The Q-PCR assay was used to detect the expressions of Nox4 mRNA and eNOS mRNA. Nitrate reduction assay was used to detect intracellulr NO levels. Results: Astragalus polysaccharide could up-regulate the expressions of p-AMPK, p-Akt, p-eNOS and eNOS mRNA in human umbilical vein endothelial cells induced with palmitic acid, down-regulate the expressions of Nox4 and Nox4 mRNA, and increase the production of NO in cells. Conclusion: Astragalus polysaccharides can promote the production of NO in endothelial cells, which can prevent and treat the damage of vascular endothelial cells. Its regulation mechanism may be associated with its ability to activate intracellular AMPK, Akt, active eNOS, and decrease the expression of Nox4.

Key words： astragalus polysaccharide vascular endothelial dysfunction nitric oxide atherosclerosis

收稿日期: 2018-06-11

基金资助:2014年温州市高层次人才创新项目（604090352/ 417）。

通讯作者: 胡臻，主任中医师，硕士生导师，Email：1435975104@qq.com。

作者简介: 胡兰兰（1991-），女，湖南衡阳人，中医师，硕士。

链接本文:

https://xb.wmu.edu.cn/CN/ 或 https://xb.wmu.edu.cn/CN/Y2018/V48/I12/876

[1] GIMBRONE M A JR, GARCÍA-CARDÑEA G. Endothelial cell dysfunction and the pathobiology of atherosclerosis[J]. Circ Res, 2016, 118(4): 620-636.
[2] ZHOU L, LIU Z, WANG Z, et al. Astragalus polysaccharides exerts immunomodulatory effects via TLR4-mediated MyD88-dependent signaling pathway in vitro and in vivo[J]. Sci Rep, 2017, 7: 44822.
[3] HANR R, TANG F, LU M, et al. Astragalus polysaccharide ameliorates H2O2-induced human umbilical vein endothelial cell injury[J]. Mol Med Rep, 2017, 15(6): 4027-4034.
[4] WEI R, MA S, WANG C, et al. Exenatide exerts direct protective effects on endothelial cells through the AMPK/Akt/eNOS pathway in a GLP-1 receptor-dependent manner[J]. Am J Physiol Endocrinol Metab, 2016, 310(11): E947-957.
[5] LIU J, ZHANG J F, LU J Z, et al. Astragalus polysaccharide stimulates glucoseuptake in L6 myotubes through AMPK activation and AS160/TBC1D4 phosphorylation[J]. Acta Pharmacol Sin, 2013, 34(1): 137-145.
[6] LU J, CHEN X, ZHANG Y, et al. Astragalus polysaccharide induces anti-inflammatory effects dependent on AMPK activity in palmitate-treated RAW264.7 cells[J]. Int J Mol Med, 2013, 31(6): 1463-1470.
[7] LU L, WANG D T, SHI Y, et al. Astragalus polysaccharide improves muscle atrophy from dexamethasone- and peroxide-induced injury in vitro[J]. Int J Biol Macromol, 2013, 61: 7-16.
[8] KONIOR A, SCHRAMM A, CZESNIKIEWICZ-GUZIK M,
et al. NADPH oxidases in vascular pathology[J]. Antioxid Redox Signal, 2014, 20(17): 2794-2814.
[9] TOUSOULIS D, KAMPOLI A M, TENTOLOURIS C, et al. The role of nitric oxide on endothelial function[J]. Curr Vasc Pharmacol, 2012, 10(1): 4-18.
[10] BARBOSA V A, LUCIANO T F, MARQUES S O, et al. Acute exercise induceendothelial nitric oxide synthase phosphorylation via Akt and AMP-activated protein kinase in aorta of rats: role of reactive oxygen species[J]. Int J Cardiol, 2013, 167(6): 2983-2988.
[11] LASSÈGUE B, GRIENDLING K K. NADPH oxidases: functions and pathologies in the vasculature[J]. Arterioscler Thromb Vasc Biol, 2010, 30(4): 653-661.
[12] HAN R, TANG F, LU M, et al. Protective effects of Astragalus polysaccharides against endothelial dysfunction in hypertrophic rats induced by isoproterenol[J]. Int Immunopharmacol, 2016, 38: 306-312.
[13] XIE L, WU Y, FAN Z, et al. Astragalus polysaccharide protects human cardiac microvascular endothelial cells from hypoxia/reoxygenation injury: The role of PI3K/AKT, Bax/Bcl-2 and caspase-3[J]. Mol Med Rep, 2016, 14(1): 904-910.
[14] KHAN M J, RIZWAN ALAM M, WALDECK-WEIERMAIR M, et al. Inhibition of autophagy rescues palmitic acid-induced necroptosis of endothelial cells[J]. J Biol Chem, 2012, 287(25): 21110-21120.
[15] LEE C H, LEE S D, OU H C, et al. Eicosapentaenoic acid protects against palmitic acid-induced endothelial dysfunction via activation of the AMPK/eNOS pathway[J]. Int J Mol Sci, 2014, 15(6): 10334-10349.
[16] CHEN X, LIU L, PALACIOS G, et al. Plasma metabolomics reveals biomarkers of the atherosclerosis[J]. J Sep Sci, 2010, 33(17-18): 2776-2783.