IGFBP-4在灯盏花素抑制肺癌A549细胞增殖中的作用

摘要
图/表
参考文献
相关文章 (0)

全文: PDF (1442 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要目的：探讨胰岛素样生长因子结合蛋白家族4（IGFBP-4）在灯盏花素（BVP）抑制肺癌A549细胞增殖中的作用。方法：培养肺癌A549细胞，用0、25、50和100 μmol/L BVP处理A549细胞48 h后，用MTT方法检测细胞存活率，台盼蓝染色法检测死亡细胞比例，Annexin V/Propidium iodide（PI）双染法检测细胞凋亡，Western blot、qRT-PCR和ELISA方法检测Bax、Bcl-2及IGFBP-4的表达和分泌情况。建立IGFBP-4高表达和低表达A549细胞，经100 μmol/L BVP处理48 h后，用MTT方法检测细胞存活率，Western blot检测Bax、Bcl-2的表达。结果：25、50和100 μmol/L BVP显著减低细胞存活，增加细胞凋亡，上调Bax表达，下调Bcl-2表达。25、50和100 μmol/L BVP上调细胞内IGFBP-4 mRNA和蛋白表达以及增加培养基中IGFBP-4含量。IGFBP-4高表达降低A549细胞存活率，增加BVP抗A549细胞增殖作用，IGFBP-4低表达增加细胞存活率，部分降低BVP抗A549细胞增殖作用。结论：BVP具有抗A549细胞增殖作用，其机制可能与其上调IGFBP-4表达有关。

	服务
	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	孙蕾
	李伟文
	黎媛
	蓝秀
	吕祝庆

关键词 ：灯盏花素, 胰岛素样生长因子结合蛋白家族4, 增殖抑制, A549细胞

Abstract：Objective: To investigate the role of IGFBP-4 in breviscapine (BVP) inhibited lung cancer A549 cells growth. Methods: A549 cells were treated with BVP 0, 25, 50 and 100 μmol/L BVP for 48 h. Adv-IGFBP-4-OE™ DNA, EGFP and Adv-KD™ Easy RNAi EGFP were used to establish IGFBP-4 over- or down- expression A549 cells. MTT assay was used to detect cell survival rate, Trypan blue staining was applied to analyze cell death rate, Annexin V/Propidium iodide (PI) double staining was used to test cell apoptosis. Western blot, RT-qPCR and ELISA were used to detect the expression Bax, Bcl-2, the protein and mRNA expression and secretion of IGFBP-4. Results: The viability decreased, but apoptosis rate increased significantly in A549 cells with 25, 50 and 100 μmol/L BVP treatment. BVP also increased Bax but reduced Bcl-2 expression. The mRNA and protein expression of IGFBP-4 and the secretion of IGFBP-4 increased with BVP treatment for 48 h. The over-expression of IGFBP-4 decreased the survival rate of A549 cells, and enhanced the growth inhibition by 100 μmol/L BVP. The down-expression of IGFBP-4 increased the cell survival rate, and alleviated the growth inhibition by 100 μmol/L BVP. Conclusion: BVP has an inhibitory effect on A549 growth, which might be related to overexpression of IGFBP-4.

Key words： breviscapine insulin-like growth factor binding protein 4 growth inhibition A549 cells

收稿日期: 2018-05-06

基金资助:浙江省科技厅公益性技术应用研究计划项目（2016c 37100）。

通讯作者: 李伟文，副主任医师，Email：2225257177@qq.com。

作者简介: 孙蕾（1976-），女，浙江丽水人，副主任医师。

链接本文:

https://xb.wmu.edu.cn/CN/ 或 https://xb.wmu.edu.cn/CN/Y2018/V48/I11/813

[1] CHEN W, ZHANG S, ZOU X. Evaluation on the incidence, mortality and tendency of lung cancer in China[J]. Thorac Cancer, 2010, 1(1): 35-40.
[2] ZHENG R, ZENG H, ZUO T, et al. Lung cancer incidence and mortality in China, 2011[J]. Thoracic Cancer, 2016, 7 (1): 94-99.
[3] MOORE B S, CARTER G T, BRÖNSTRUP M. Editorial: Are natural products the solution to antimicrobial resis-tance?[J]. Nat Prod Rep, 2017, 34(7): 685-686.
[4] ABDELMOHSEN U R, BALASUBRAMANIAN S, OELSCHLAEGER T A, et al. Potential of marine natural products against drug-resistant fungal, viral, and parasitic infec-tions[J]. Lancet Infect Dis, 2017, 17(2): e30-e41.
[5] WU Y, FAN Q, LU N, et al. Breviscapine-induced apoptosis of human hepatocellular carcinoma cell line HepG2 was involved in its antitumor activity[J]. Phytother Res, 2010, 24 (8): 1188-1194.
[6] WANG Q, WANG Q, WANG S F, et al. Oral Chinese herbal medicine as maintenance treatment after chemotherapy for advanced non-small-cell lung cancer: a systematic review and meta-analysis[J]. Curr Oncol, 2017, 24(4): e269-e76.
[7] ZENG J, CAI S. Breviscapine suppresses the growth of non-small cell lung cancer by enhancing microRNA-7 expres-sion[J]. J Biosci, 2017, 42(1): 121-129.
[8] KE Y, BAO T, WU X, et al. Scutellarin suppresses migration and invasion of human hepatocellular carcinoma by inhibiting the STAT3/Girdin/Akt activity[J]. Biochem Biophys Res Commun, 2017, 483(1): 509-515.
[9] GUAN Y B, YANG D R, NONG S J, et al. Breviscapine (BVP) inhibits prostate cancer progression through damaging DNA by minichromosome maintenance protein-7 (MCM-7) modulation[J]. Biomed Pharmacother, 2017, 93: 103-116.
[10] DAMON S E, MADDISON L, WARE J L, et al. Overexpression of an inhibitory insulin-like growth factor binding protein (IGFBP), IGFBP-4, delays onset of prostate tumor formation[J]. Endocrinology, 1998, 139(8): 3456-3464.
[11] DURAI R, YANG S Y, SEIFALIAN A M, et al. Role of insulin-like growth factor binding protein-4 in prevention of colon cancer[J]. World J Surg Oncol, 2007, 5: 128.
[12] XIAO Y, ZHU S, YIN W, et al. IGFBP-4 expression is adversely associated with lung cancer prognosis[J]. Oncol Lett, 2017, 14(6): 6876-6880.
[13] SATO H, SAKAEDA M, ISHII J, et al. Insulin-like growth factor binding protein-4 gene silencing in lung adenocarci-nomas[J]. Pathol Int, 2011, 61(1): 19-27.
[14] LI W, SUN D, LV Z, et al. Insulin-like growth factor binding protein-4 inhibits cell growth, migration and invasion, and downregulates COX-2 expression in A549 lung cancer cells [J]. Cell Biol Int, 2017, 41(4): 384-391.