CAO Yanjing,WU Jiali,LI Peng, et al. The expression analysis of transmitter related vesicle transporters of hippocampal in CCI induced-vascular dementia rats[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2018, 48(11): 801-806,812.
Abstract：Objective: To investigate the expression of different vesicles transporter (different synapses) vGluTs, vAChT and vGAT in the hippocampal CA1-3 region in rats with chronic global cerebral ischemia. Methods: VaD animal model was established by BCCAO method, Western blot and immunofluorescence were used to detect the expression of vGluTs, vAChT and vGAT in hippocampal CA1-3. Results: In the Morris test, the escape latency of VaD rats was significantly extended compared with sham and control animals (P<0.05). The percentage of searching time in the VaD group was significantly reduced (P<0.05) and the searching distance in target quadrant was significantly decreased (P<0.01). In the T-maze test: the error rate of the delayed operation of VaD rats was significantly increased (P<0.01). The levels of vGluT1 and vGluT3 in the hippocampal CA1-3 region of VaD rats were significantly lower than those in the sham and control groups (P<0.01). Compared with the sham and control groups, the expression of vAChT in the hippocampal CA1-3 region of VaD rats was decreased (P<0.01). Compared with sham and control groups, the number of vGluT1 and vGluT3 positive neurons in VaD rats decreased in hippocampal CA1-3 region, and the number of vAChT positive neurons in hippocampal CA1-3 was also significantly decreased (P<0.01). Conclusion: Glutamate, cholinergic vesicle cargo function defects, glutamate, central cholinergic neurotransmitter imbalance, neuronal loss, CA1-CA3 hippocampus information loop destruction is the main cause of cognitive dysfunction in rats under the ischemic conditions.
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