Analysis of Nrf2 expression and oxidative stress in the placental, uterine and cardiac tissues of rats with gestational diabetes mellitus
SUN Congcong1,2, ZHENG Jiayong1,2, WANG Wenhuan1,2, HU Yanjun1, ZHENG Jianqiong1, ZHANG Hongping1
1.Department of Gynecology and Obstetrics, Wenzhou People’s Hospital, Wenzhou Maternal and Child Health Care Hospital, Wenzhou, 325000; 2.Wenzhou City Key Laboratory of Gynecology and Obstetrics, Wenzhou, 325000
SUN Congcong,ZHENG Jiayong,WANG Wenhuan, et al. Analysis of Nrf2 expression and oxidative stress in the placental, uterine and cardiac tissues of rats with gestational diabetes mellitus[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2018, 48(11): 796-800.
Abstract：Objective: To analyze the varied expressions of Nrf2 and the level of oxidative stress in the placental, uterine and cardiac tissues from gestational diabetes mellitus (GDM) rats and gestational rats. Methods: Twenty pregnant SD rats were randomly divided into GDM group and normal group. GDM group rats were injected with streptozotocin intraperitoneally to induce GDM models, while rats in the control group were injected with the same amount of citric acid buffer intraperitoneally. All the rats were sacrificed at day 30 of pregnancy by anesthesia, and the placentae, uteruses and hearts were excised for protein extraction. Subsequently, the levels of insulin, SOD as well as GSH in different tissues were assayed by ELISA. The level of MDA was examined by the thiobarbital acid colorimetry. The expression level of Nrf2 was analyzed by Western blot. Results: The insulin and MDA from the placental, uterine tissues were higher in GDM group compared with the normal group (P<0.05), while GSH and SOD were lower (P<0.05). Also, the GDM rats exhibited higher Nrf2 protein level in the placental and uterine tissues compared with normal group (P<0.05). However, there was no significant difference in cardiac tissue between the two groups (P>0.05). Conclusion: GDM leads to insulin resistance, oxidative stress and the elevated Nrf2 expression in the placenta and uterine, which are unaffected in the hearts. Thus, it is speculated that GDM-induced oxidative stress and Nrf2 alteration differ among tissues.
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