1. Department of Gastroenterology, the Affiliated Hospital of Medical School of Ningbo University, Ningbo, 315020; 2.Department of Biochemistry and Molecular Biology, Medical School of Ningbo University, Zhejiang Province Key Laboratory of Pathophysiology Technology Research, Ningbo, 315211
ZHOU Yuping,LYU Xueyou,QU Hui, et al. The circRNA expression profile in the tissue of liver fibrosis mice[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2018, 48(8): 563-566.
Abstract：Objective: To study the expression profile of circular RNA (circRNA) in hepatic tissues in a liver fibrosis model. Methods: The classic CCl4 mouse liver fibrosis model was established alongside a normal control group. The high-throughput circRNA microarray technique was used to compare the changes of circRNA expression profiles in the liver of the two groups. The target circRNA was selected according to the principle of high expression difference and good consistency between samples, RT-qPCR was used to verify its expression in liver tissue, and bioinformatics software was used to predict microRNA (miRNAs) which may be regulated by it. Results: There were 69 circRNAs differentially expressed (P<0.05) in liver tissues between the normal group and the model group, with the difference being more than 2 times. Among them, one was up-regulated by more than 4 times in the model group and five were down-regulated by more than 4 times. RT-qPCR experiments confirmed that the five circRNA trends were consistent with the microarray results. Four of them were statistically different (P<0.05). Bioinformatics analysis combined with literature retrieval showed that mmu_circ_42398, mmu_circ_42397 could combine with miR-338-3p, while mmu_circ_34116 could combine with miR-141-5p in their participation in the pathogenesis of liver fibrosis. Conclusion: The circRNA expression profile in liver fibrosis tissues shows significant changes and circRNA may be involved in the pathogenesis of liver fibrosis.
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