YE Xiaoxian,MAO Shanshan,SONG Yiling, et al. Prediction and analysis of B cell epitopes of type HPV58 E6 protein[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2018, 48(8): 547-551.
Abstract：Objective: To predict and analyze the B cell linear epitopes of the high-risk human papillomavirus HPV 58 E6 protein and its structure. Methods: E6 protein sequences retrieved from the Swissprot protein database provided by the Swiss Institute of bioinformatics were analyzed using computer aided bioinformatics software, and Expasy Server analysis was used to predict physicochemical properties, secondary structure, three-dimensional structure of E6 protein. DNASTAR software was used to further analyze its hydrophilicity, flexibility, surface probability, antigenicity and polarity and other characteristics. Combined with the amino acid antigen index calculation method, the average antigen index (AI) was calculated, and according to the AI size, the potential peptide of B cell linear epitopes of HPV 58 E6 protein was predicted and analyzed by homology matching. Results: The B cell linear epitopes of HPV58 E6 protein may be located at the following peptide position: N-terminal 8-15 (KPRTLHDL), 27-44 (ELKCVECKKTLQRSEVYD), 108-127 (RPLCPQEKKRHVDLNKRFHN), 141-147 (RPRRRQT) peptide and its surrounding. The homology analysis showed that peptide 27-44 and 141-147 are possible linear epitopes of B cells, which are unique sequences of cell type HPV58 E6 B epitopes. The homology match between peptide 8-15 and HPV33 type E6 protein was 100%. The peptide 108-127 matches HPV33 type E6 and HPV9 E6 protein being 100% homologous. Conclusion: 27-44, 141-147 peptide are the potential dominant epitopes of B protein of HPV58 type E6.
