生存素对淀粉样前体蛋白代谢的调节作用

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摘要目的：探索生存素（survivin）对淀粉样前体蛋白（APP）代谢的调节作用。方法：通过免疫荧光技术，利用激光共聚焦显微镜观察体外原代培养的大鼠海马神经元内survivin与APP是否存在共定位。进而通过APP质粒单转染或APP和survivin质粒共转染细胞，并利用Western blot方法检测survivin是否影响APP的表达。将前述的转染细胞进行凋亡染色后，经流式细胞仪检测，比较suivivin是否可以抑制APP引起的细胞凋亡。结果：体外原代培养的大鼠海马神经元内survivin与APP可以共存，survivin可以抑制APP在PC12细胞内的表达，APP过量表达可以诱发细胞凋亡，survivin可以抑制APP所引起的细胞凋亡。结论：Survivin可以抑制APP在细胞内的表达，进而抑制APP过表达引起的细胞凋亡。

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	蒋伟青
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关键词 ：生存素, 淀粉样前体蛋白, 细胞凋亡

Abstract：Objective: To explore the regulatory effect of survivin in amyloid precursor protein (APP) metabolism. Methods: Immunofluorescence technique was conducted to observe whether there was colocalization of survivin and APP through confocal imaging. Cell transfection with APP or APP-survivin plasmids were conducted to detect: whehter survivin affected the expression of APP through western blot analysis, and whether survivin inhibited the cell apoptosis induced by overexpression of APP though flow cytometry. Results: Survivin colocalized with APP in cultured rat primary hippocampal neurons. Survivin inhibited the expression of APP in PC12 cells. Moreover, survivin inhibited the cell apoptosis induced by overexpression of APP. Conclusion: Survivin could inhibit the cellular protein expression of APP and could inhibit the cell apoptosis induced by overexpression of APP.

Key words： survivin amyloid precursor protein apoptosis

收稿日期: 2018-01-24

基金资助:国家自然科学基金资助项目（31770800，81571329）；上海市教委——高峰临床医学“研究型医师”项目（20161414）。

通讯作者: 王永刚，主任医师，Email：wangyg@shsmu.edu.cn

作者简介: 蒋伟青（1992-），女，上海人，硕士生。

链接本文:

https://xb.wmu.edu.cn/CN/ 或 https://xb.wmu.edu.cn/CN/Y2018/V48/I7/495

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