WANG Yonghui1,MAO Weibo2,LOU Jian1, et al. Expression of CD44V6, p-Src and Ki67 and their correlation in gastric cancer[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2014, 44(4): 272-277.
Abstract：Objective: To study the expression of the cancer stem cell marker CD44V6, non-receptor tyrosine kinase p-Src and Ki67 proliferation index in gastric cancer and paracancer tissues, analyze their relationship with clinicopathological parameters and prognosis of gastric cancer, and further explore the possible link between the three. Methods: Immunohistochemical staining (EnvisionTM) was used to detect CD44V6, p-Src (Y419) and Ki67 in 85 cases of gastric carcinoma and 41 cases of corresponding paracancer tissues. Differences in the expression of the three and the association with the clinicopathological parameters were analyzed. The possible links that exist between the three were explored. Results: The expression rate of CD44V6, p-Src (Y419) and Ki67 in the gastric cancer tissues was significant higher than that in the adjacent non-tumorous tissues. The expression of CD44V6 was significantly related with tumor size, depth of invasion and pTNM classification (P<0.05); The high expression of p-Src (Y419) was significantly related with tumor size, tumor differentiation and depth of invasion (P<0.05). The high expression of Ki67 was significantly related with tumor size, Lauren classification (P<0.05). Univariate survival analysis revealed a significant association between lower 2-years survival time and CD44V6 expression, p-Src (Y419) and Ki67 high expression. Multivariate analysis revealed that CD44V6 expression and elevated p-Src (Y419) and Ki67 expression were not an independent poorly prognostic parameter for 2-years survival time. The expression of p-Src and Ki67 was higher in the CD44V6 positive expression gastric cancer tissues than that in CD44V6 negative expression gastric cancer tissues. There was positive correlation among CD44V6, p-Src and Ki-67 expression (P<0.05). Conclusion: The expression of CD44V6, p-Src and Ki67 maybe a biological marker for poor prognosis for gastric cancer.
