LIU Yunxiao,PAN Xiaoqiong,HU Zhen.. Effect of dihydromyricetin against H2O2 induced injury in human umbilical vein endothelial cell[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2014, 44(4): 264-267.
Abstract：Objective: To investigate the effects of pretreatment with DMY on H2O2 induced damage in human umbilical vein endothelial cell (HUVECs) and to explore the related mechanism. Methods: Groups were set as below: normal control group, H2O2 group, groups for low, medium and high dosage of dihydromyricetin (DMY) respectively.Different concentrations of DMY (5μg·mL-1, 20μg·mL-1, 80μg·mL-1) precultured HUVECs for 12 hours,then 0.5 mmol·L-1 H2O2 was used to invervene for 12 hours, using MTT to detect cell viability, Hoechst33258 for apoptotic nuclear staining, the supernatant was taken to determine SOD activity and MDA content. Flow cytometry was used to determine intracellular reactive oxygen species (ROS) and calcium levels. Results: DMY could significantly protect H2O2 (0.5 mmol·L-1) after H2O2-induced injury in HUVECs (P<0.05). We found SOD activity in the supernatant of increased while MDA content and intracellular ROS expression including calcium concentration all decreased of which results became obvious when in 5μg·mL-1 solution of DMY. Conclusion: DMY can reduce intracellular ROS expression through intracellular Ca2+ pathway, DMY concentration of 5μg·mL-1 has the most obvious effect.
