YU Yaojun,SHENG Weiwei,YE Haibo, et al. The mechanism of elemene and PD98059 inducing apoptosis of human gastric cancer cell line SCG- 7901[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2014, 44(4): 258-263.
Abstract：Objective: To explore the effect of elemene and PD98059 on the apoptosis of human gastric cancer cell line SGC-790l and the relationship of ERK1/2, P38MAPK signal pathway. Methods: SGC-790l cells were treated with elemene and PD98059. MTT assay was used to detect the proliferation of SGC-790l cells. The expression of ERK1/2, phosphorylation REK1/2 (p-ERK1/2) and phosphorylated P38 (p-P38) was detected by western-blot.The bcl-2 mRNA and bax mRNA was detected with RT-PCR. The gastric cancer cell apoptosis index was detected by TUNEL method. Results: Elemene and PD98059 alone effectively inhibited the proliferation of gastric cancer cells SGC-7901, and the former was in a dose-dependent and time-dependent manner, the latter was in a time-dependence but no in dose-dependent manner, and the combined effects of the two drugs on gastric cancer cell proliferation inhibition was significantly greater than that of single agent (P<0.05). The expression of p-ERK1/2 protein was increased (P<0.05), and the total ERK1/2 was no significant change with the concentration of elemene increased. The expression of p-P38 of elemene group, PD98059 group and the combined group were significantly higher than that of the control group (P<0.05), and the combined group was the highest (P<0.05). The mRNA of bcl-2 expression was down-regulated while the expression of bax mRNA was up-regulated and both changes had good dose-dependent tendency, and the combined group was most significantly. Cell in experimental groups apoptosis rate was higher than that in the control group (P<0.05), and in a dose-dependent manner with elemene (P<0.05).The apoptosis rate of elemene+PD98059 group was significantly higher than that of the single-action group (P<0.05). Conclusion: Elemene and PD98059 can inhibite the proliferation of gastric cancer cells SGC-7901, and the former was in a dose-dependent and time-dependent manner. Elemene and PD98059 can also promote the apoptosis of gastric cancer cells. Elemene promotes apoptosis and inhibites the proliferation of gastric cancer cells by up-regulating the expression of p-ERK1/2 signaling pathway and p-P38MAPK signaling pathway. PD98059 inhibites the ERK1/2 phosphorylation, but can promote the expression of p-P38 MAPK signaling pathway, then inhibites the proliferation and promotes gastric cancer cell apoptosis.
