Comparative study between guinea pig ovarian fragments and isolated preantral follicles cryopreservation with vitrification
1.The Reproductive Medicine Centre, the First Affiliated Hospital of Guangxi Medical University, Nanning, 530021; 2.Centre for Reproductive Medicine, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015; 3.Monash Institute of Medical Research, Monash University,Clayton, 3800
ZHANG Yue1,CHEN Wenming2,XIE Chichi2, et al. Comparative study between guinea pig ovarian fragments and isolated preantral follicles cryopreservation with vitrification[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2014, 44(4): 252-257.
Abstract：Objective: To compare the follicle morphology, survival rate and in vitro growth potential of guinea pig ovarian fragments and isolated preantral follicles after vitrification. Methods: Five- weeks aged female guinea pigs were used. Two vitrification solutions (solution A and B) were applied to vitrify guinea pig ovarian fragments and isolated preantral follicles. The toxicity and cryopreservation efficiency of the two solutions were tested on both fragments and isolated preantral follicles. The ovaries were observed using hematoxylin and eosin staining; the viability of the follicles was evaluated by trypan blue. The isolated preantral follicle was cultured in vitro to evaluated the follicle development potential. Results: Based on morphological analyze, the majority follicles were normal in fragments cryopreservation, but the abnormal follicle were higher than fresh group, P<0.05. The recover rates of follicle after being thawed using trypan blue were 66.1% in solution A fragment vitrification group, significantly lower than that in solution B follicle vitrification group (78.5%) and fresh group (87.5%), P<0.05. No significance was found among other groups. During in vitro culture, the vitrified fragments showed poorer develop potential than follicle vitrification groups, typically solution A fragment group, and the follicle survival rate by day 6 was lower than (20.0%), significantly lower than fresh group (60.0%) and solution B follicle vitrification group (50.0%), P<0.05. Conclusion: The results show that the vitrification can preserve guinea pig ovarian fragments and isolated preantral follicles, the latter one achieved better results. Solution B was more efficient in preserving guinea pig follicles. The follicles show growth potential during in vitro culture after being thawed.
