WU Wenzhi,XU Ying,WU Jinming, et al. Effects of HBV S-ecdCD40L on phenotype and function of dendritic cells from hepatitis B virus genome transgenic mouse [J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2014, 44(4): 248-.
Abstract：Objective: To examine the influence of expression of HBV S-ecdCD40L on the maturity differentiation and function of the dendritic cells (DC) from hepatitis B virus (HBV) genome transgenic mouse models, and to explain the possible mechanism. Methods: HBV transgenic mice were injected with pcDNA3.1-S-ecdCD40L, pcDNA3.1-S, pcDNA3.1 and PBS respectively, (each 100μg/25 g). Vaccination was performed on, day 15 and day 30. Six weeks after first injection sera were collected. Isolate DC and examine the phenotypic and functional changes, and the HBV DNA copies in HBV transgenic mice serum were detected by real-time PCR. Results: Compared with other three control groups, DC from injection with pcDNA3.1-S-ecdCD40L mice enhanced expression of costimulatory molecules (CD80, CD86, MHC II), Tollrike receptor 4 (TLR4), proinflammatory cytokine (IL-12p70) and also the capacity to induce allogeneic lymphocytes proliferation. And the average copies of serum HBV DNA were lower than those of the other three control groups. Conclution: The study in vivo supports the concept that the recombinant plasmids pcDNA 3.1-S-ecdCD40L-mediated HBV S-ecdCD40L expression may be a useful strategy for activating DC抯 maturity and enhancing its functions.
