Effects of IFN-Y on the expression of transcription factors GATA3 in rat pulmonary fibrosis
TU Junwei1，ZHA0 Jianping1．ZHU Jingqian1．SHENG Lin1．ZHANG Yili2
1．Department ofRespiration．Jinhua Central Hospital，Jinhua，321000；2．Intensive Care Unit，the Second Affiliated Hospital of Wenzhou Medical College，Wenzhou，325000
Abstract：Objective：To explore the effects of IFN—v on the expression of transcription factors GATA3 in rat pulmonary fibrosis．Methods：Male SD rats were randomly divided into three groups：the control group(C)，bloemycin groups(group BLM)andIFN—Y treated group(group BLM—in)．The pulmonary fibrosis model was established by intraperitoneal injection with bloemycin(15 mrdkg X 10 day)．Rats in BLM and BLM—IFN group were randomly sacrificed on 7山day(group BLMl and BLM—IFNl)，14山day(group BLM2 and BLM-IFN2)and 280 day(group BLMd and BLM—IFN4)，respectively。Semi—quantitative analysis of the histopathologic changes stained with Haematoxylin-eosin(H-E)was performed，and the mRNA expression of transcription factors GATA3 in each group was measured with RT-PCR．Results：①alveolitis was the primarily pathologic change of lung tissue in BLMl and BLM·IFNl，while alveolitis and fibrosis in BLM2 and fibrosis in BLM4．Obviously alleviated fibrosis was seen in both BLM—IFN，and BLM—IFN4 group．②As compared with group C，the expression of GATA3 gene was significantly higher in each bleomycin group，(P<0．0 l respectively)．After treatment with IFN-V，the expression of GATA3 gene in BLM—INFl，BLM-INF2 and BLM·INF4 was lower than that in counterpart group of BLMs．(P<O．01 respectively)，even lower than that in group C．Conclusion：IFN—v treatment can reduce the degree of rat pulmonary fibrosis induced by bloemycin．The mechanism may be that the IFN—v can inhibit the expression of GATA3 gene，and reverse the imbalance of Th l／Th2．
