胚胎干细胞分泌因子增强AML-12细胞抗凋亡能力

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摘要目的：研究胚胎干细胞分泌因子是否能增强AML-12细胞在恶劣条件培养下的抗凋亡能力。方法：分别用胚胎干细胞完全培养基（CON-M）和胚胎干细胞上清液（ESC-M）培养AML-12细胞，并置于恶劣细胞培养环境中培养，MTT法比较各组AML-12细胞活力；Seahorse细胞代谢分析仪比较各组AML-12细胞氧消耗率以及细胞外酸化率等呼吸功能代谢情况。结果：MTT结果显示在恶劣细胞培养环境中，于ESC-M中培养的AML-12细胞拥有更强的抗凋亡能力。Seahorse细胞代谢分析显示ESC-M培养能加强AML-12细胞糖酵解潜力、最大氧化呼吸能力以及氧化呼吸潜力。结论：胚胎干细胞分泌因子能够增强AML-12细胞在恶劣培养条件下的抗凋亡能力。

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关键词 ：胚胎干细胞, 细胞生存, 肝细胞, 代谢, AML-12细胞

Abstract：Objective: To investigate whether the embryonic stem cell secreted cytokines can enhance AML-12 cell anti-apoptotic ability in extreme cell culture conditions. Methods: AML-12 cells were respectively treated with embryonic stem cells complete media (CON-M) or embryonic stem cells conditioned media (ESC-M) and cultured in extreme cell culture conditions. Cells viability was detected by MTT assay. Mitochondrial energy metabolism including oxygen consumption rate and extracellular acidification rate were measured using a Seahorse metabolic flux analyzer. Results: MTT assay indicated that AML-12 cells treated with ESC-M had stronger anti-apoptotic abilities in extreme cell culture conditions. Results from the Seahorse metabolic flux analyzer showed that ESC-M could increase AML-12 cells maximal respiration capacity, spare respiration capacity as well as glycolytic capacity. Conclusion: The embryonic stem cells secreted cytokines enhance AML-12 cells anti-apoptotic abilities in extreme cell culture conditions.

Key words： embryonic stem cell cell survival hepatocyte metabolism AML-12 cells

收稿日期: 2018-01-18

基金资助:国家自然科学基金资助项目（80215021）。

通讯作者: 陈肖鸣，主任医师，教授，硕士生导师，Email：cxm@wmu.edu.cn。

作者简介: 林武（1992-），男，浙江温州人，硕士生。

链接本文:

https://xb.wmu.edu.cn/CN/ 或 https://xb.wmu.edu.cn/CN/Y2018/V48/I6/408

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