长链非编码RNA FENDRR在子宫颈癌中的作用

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摘要目的：探讨长链非编码RNA FENDRR在子宫颈癌（下面简称宫颈癌）中的作用。方法：在宫颈癌细胞系中使用siRNA干扰技术特异性干扰FENDRR的表达，用qRT-PCR检测siRNA的干扰效率；运用CCK-8和Transwell实验分别检测干扰FENDRR表达对宫颈癌细胞体外增殖和运动能力的影响；运用裸鼠成瘤实验验证干扰FENDRR表达对宫颈癌细胞体内成瘤能力的影响。结果：siRNA转染使FENDRR在SiHa和HeLa细胞中的相对表达量分别由（1.013±0.120）、（1.001±0.025）降为（0.226±0.024）、（0.099±0.010），差异均有统计学意义（P＜0.01）；干扰FENDRR后宫颈癌细胞系的增殖能力明显增强（P＜0.01），迁移和侵袭能力也显著增强（P＜0.01）；干扰FENDRR后宫颈癌细胞HeLa在裸鼠体内的成瘤能力明显增强，与对照组相比肿瘤体积从（853.2±59.4）mm3增加到（1 155.0±51.5）mm3（P＜0.01），肿瘤的质量从（0.544±0.053）g增加到（0.814± 0.061）g（P＜0.05）。结论：干扰FENDRR的表达明显增强宫颈癌细胞的体外增殖和运动能力以及体内成瘤能力，FENDRR可能在宫颈癌的进展中发挥重要的抑癌作用。

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关键词 ：长链非编码RNA, FENDRR基因, 宫颈肿瘤, RNA, 小分子干扰, 细胞增殖, 细胞运动, 裸鼠成瘤

Abstract：Objective: To investigate the effects of long non-coding RNA FENDRR in cervical cancer. Methods: Cervical cancer cells were transfected with siRNA that specifically targeting FENDRR. QRT-PCR was used to verify the transfection effect of siRNA. Proliferation and motility capacities of cervical cancer cells were demonstrated by CCK-8 and Transwell assays, respectively. Tumorigenesis in nude mice was performed to identify the proliferation ability of cervical cancer cell in vivo. Results: With siRNA transfection, the expression levels of FENDRR in SiHa and HeLa were significantly reduced from (1.013±0.120), (1.001±0.025) to (0.226± 0.024), (0.099±0.010) (P<0.01). After transfecting with siRNA targeting FENDRR, the capacity of proliferation was significantly enhanced in cervical cancer cells (P<0.01), as well as migration and invasion abilities (P<0.01). The tumorigenesis in nude mice was obviously improved by interfering FENDRR expression in HeLa. Compared with the control group, the tumor volume increased from (853.2±59.4) mm3 to (1 155.0±51.5) mm3 (P<0.01), the tumor weight increased from (0.544±0.053) g to (0.814±0.061) g (P<0.05). Conclusion: Knockdown the expression of FENDRR in cervical cancer cells significantly enhanced their proliferation, migration as well as invasion abilities in vitro, and improved their tumorogenesis in vivo, which indicated that FENDRR may play an important inhibitory role in the progression of cervical cancer.

Key words： long non-coding RNA FENDRR gene uterine cervical neoplasms RNA, small interfering cell proliferation cell movement tumorigenesis

收稿日期: 2017-07-24

基金资助:浙江省公益技术应用计划项目（2016C37132）；浙江省自然科学基金资助项目（LY17H040009）。

通讯作者: 吴雪清，主任医师，教授，硕士生导师，Email：wuxueqing.37@hotmail.com

作者简介: 兰霄霄（1992-），女，浙江温州人，硕士生。

链接本文:

https://xb.wmu.edu.cn/CN/ 或 https://xb.wmu.edu.cn/CN/Y2018/V48/I4/262

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